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Number of items: 69.
A
Adeyelu, Tolulope;
Bordin, Nicola;
Waman, Vaishali P;
Sadlej, Marta;
Sillitoe, Ian;
Moya-Garcia, Aurelio A;
Orengo, Christine A;
(2023)
KinFams: De-Novo Classification of Protein Kinases Using CATH Functional Units.
Biomolecules
, 13
(2)
, Article 277. 10.3390/biom13020277.
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B
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Bates, George Thomas;
(2023)
Molecular mechanisms regulating CHC22 clathrin function.
Doctoral thesis (Ph.D), UCL (University College London).
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Bdzhola, AV;
Filonenko, VV;
Gout, IT;
Malanchuk, OM;
(2023)
The use of the in situ proximity ligation assay for validating S6 kinase 1 CoAlation under oxidative stress.
Biopolymers & Cell
, 39
(4)
pp. 277-282.
10.7124/bc.000AA5.
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Blacker, Thomas S;
Duchen, Michael R;
Bain, Angus J;
(2023)
Distinct NAD(P)H binding configurations revealed by fluorescence lifetime, anisotropy and polarised two-photon absorption.
Biophysical Journal
10.1016/j.bpj.2023.02.014.
(In press).
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Bolik-Coulon, N;
Sever, AIM;
Harkness, RW;
Aramini, JM;
Toyama, Y;
Hansen, DF;
Kay, LE;
(2023)
Less is more: A simple methyl-TROSY based pulse scheme offers improved sensitivity in applications to high molecular weight complexes.
Journal of Magnetic Resonance
, 346
, Article 107326. 10.1016/j.jmr.2022.107326.
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Bordin, Nicola;
Lau, Andy M;
Orengo, Christine;
(2023)
Large-scale clustering of AlphaFold2 3D models shines light on the structure and function of proteins.
Molecular Cell
, 83
(22)
pp. 3950-3952.
10.1016/j.molcel.2023.10.039.
|
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Bordin, Nicola;
Sillitoe, Ian;
Nallapareddy, Vamsi;
Rauer, Clemens;
Lam, Su Datt;
Waman, Vaishali P;
Sen, Neeladri;
... Orengo, Christine; + view all
(2023)
AlphaFold2 reveals commonalities and novelties in protein structure space for 21 model organisms.
Communications Biology
, 6
(1)
, Article 160. 10.1038/s42003-023-04488-9.
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Boron, Veronica A;
Martin, Andrew CR;
(2023)
abYpap: Improvements to the Prediction of Antibody V H/V L Packing Using Gradient Boosted Regression.
Protein Engineering, Design and Selection
, Article gzad021. 10.1093/protein/gzad021.
(In press).
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Bunney, Tom D;
Katan, Matilda;
(2023)
Targeting G-CSF to treat autoinflammation.
Nature Immunology
, 24
pp. 736-737.
10.1038/s41590-023-01474-5.
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C
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Caswell, Deborah R;
Gui, Philippe;
Mayekar, Manasi K;
Law, Emily K;
Pich, Oriol;
Bailey, Chris;
Boumelha, Jesse;
... Swanton, Charles; + view all
(2023)
The role of APOBEC3B in lung tumor evolution and targeted cancer therapy resistance.
Nature Genetics
10.1038/s41588-023-01592-8.
(In press).
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Charoonnart, Patai;
Taunt, Henry Nicolas;
Yang, Luyao;
Webb, Conner;
Robinson, Colin;
Saksmerprome, Vanvimon;
Purton, Saul;
(2023)
Transgenic Microalgae Expressing Double-Stranded RNA as Potential Feed Supplements for Controlling White Spot Syndrome in Shrimp Aquaculture.
Microorganisms
, 11
(8)
, Article 1893. 10.3390/microorganisms11081893.
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Chaves-Arquero, Belén;
Collins, Katherine M;
Abis, Giancarlo;
Kelly, Geoff;
Christodoulou, Evangelos;
Taylor, Ian A;
Ramos, Andres;
(2023)
Affinity-enhanced RNA-binding domains as tools to understand RNA recognition.
Cell Reports Methods
, 3
(6)
, Article 100508. 10.1016/j.crmeth.2023.100508.
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Clasen, Frederick;
Nunes, Patrícia M;
Bidkhori, Gholamreza;
Bah, Nourdine;
Boeing, Stefan;
Shoaie, Saeed;
Anastasiou, Dimitrios;
(2023)
Systematic diet composition swap in a mouse genome-scale metabolic model reveals determinants of obesogenic diet metabolism in liver cancer.
iScience
, 26
(2)
, Article 106040. 10.1016/j.isci.2023.106040.
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Crescioli, Silvia;
Correa, Isabel;
Ng, Joseph;
Willsmore, Zena N;
Laddach, Roman;
Chenoweth, Alicia;
Chauhan, Jitesh;
... Karagiannis, Sophia N; + view all
(2023)
B cell profiles, antibody repertoire and reactivity reveal dysregulated responses with autoimmune features in melanoma.
Nature Communications
, 14
(1)
, Article 3378. 10.1038/s41467-023-39042-y.
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D
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D’Halluin, Alexandre;
Polgar, Peter;
Kipkorir, Terry;
Patel, Zaynah;
Cortes, Teresa;
Arnvig, Kristine B;
(2023)
Premature termination of transcription is shaped by Rho and translated uORFS in Mycobacterium tuberculosis.
iScience
, 26
(4)
, Article 106465. 10.1016/j.isci.2023.106465.
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Dawson, Charmian Nicole;
(2023)
Disease-causing mutations in the human metal ion transporter ZIP14 alter its structure, ion uptake function and trafficking.
Doctoral thesis (Ph.D), UCL (University College London).
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Di Rocco, Giulia;
Taunt, Henry N;
Berto, Marcello;
Jackson, Harry O;
Piccinini, Daniele;
Carletti, Alan;
Scurani, Giulia;
... Purton, Saul; + view all
(2023)
A PETase enzyme synthesised in the chloroplast of the microalga Chlamydomonas reinhardtii is active against post-consumer plastics.
Scientific Reports
, 13
(1)
, Article 10028. 10.1038/s41598-023-37227-5.
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E
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Engrola, Filipa;
Correia, Márcia AS;
Watson, Cameron;
Romão, Carlos C;
Veiros, Luis F;
Romão, Maria João;
Santos-Silva, Teresa;
(2023)
Arsenite oxidase in complex with antimonite and arsenite oxyanions - insights into the catalytic mechanism.
Journal of Biological Chemistry
, Article 105036. 10.1016/j.jbc.2023.105036.
(In press).
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Esteras, Noemí;
Blacker, Thomas S;
Zherebtsov, Evgeny A;
Stelmashuk, Olga A;
Zhang, Ying;
Wigley, W Christian;
Duchen, Michael R;
... Abramov, Andrey Y; + view all
(2023)
Nrf2 regulates glucose uptake and metabolism in neurons and astrocytes.
Redox Biology
, 62
, Article 102672. 10.1016/j.redox.2023.102672.
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F
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Filonenko, V;
Gout, I;
(2023)
Discovery and functional characterisation of protein CoAlation and the antioxidant function of coenzyme A.
BBA Advances
, 3
, Article 100075. 10.1016/j.bbadva.2023.100075.
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G
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Gao, X;
Thrush, JW;
Gor, J;
Naismith, JH;
Owens, RJ;
Perkins, SJ;
(2023)
The solution structure of the heavy chain–only C5-Fc nanobody reveals exposed variable regions that are optimal for COVID-19 antigen interactions.
Journal of Biological Chemistry
, 299
(11)
, Article 105337. 10.1016/j.jbc.2023.105337.
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Gardner, S;
Darrow, MC;
Lukoyanova, N;
Thalassinos, K;
Saibil, HR;
(2023)
Structural basis of substrate progression through the bacterial chaperonin cycle.
Proceedings of the National Academy of Sciences of the United States of America
, 120
(50)
, Article e2308933120. 10.1073/pnas.2308933120.
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Goldtzvik, Yonathan;
Sen, Neeladri;
Lam, Su Datt;
Orengo, Christine;
(2023)
Protein diversification through post-translational modifications, alternative splicing, and gene duplication.
Current Opinion in Structural Biology
, 81
, Article 102640. 10.1016/j.sbi.2023.102640.
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Gong, Grace Q;
Bilanges, Benoit;
Allsop, Ben;
Masson, Glenn R;
Roberton, Victoria;
Askwith, Trevor;
Oxenford, Sally;
... Vanhaesebroeck, Bart; + view all
(2023)
A small-molecule PI3Kα activator for cardioprotection and neuroregeneration.
Nature
, 618
pp. 159-168.
10.1038/s41586-023-05972-2.
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H
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Halpern, Aaron;
Bartsch, Lilly R;
Ibrahim, Kaan;
Harrison, Stuart A;
Ahn, Minkoo;
Christodoulou, John;
Lane, Nick;
(2023)
Biophysical Interactions Underpin the Emergence of Information in the Genetic Code.
Life
, 13
(5)
, Article 1129. 10.3390/life13051129.
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Hassi, Niina K;
Weston, Timir;
Rinaldi, Giulia;
Ng, Joseph C;
Smahi, Asma;
Twelves, Sophie;
Davan-Wetton, Camilla;
... Capon, Francesca; + view all
(2023)
in-silico and in-vitro analysis of IL36RN mutations reveals critical residues for the function of the interleukin-36 receptor complex.
Journal of Investigative Dermatology
10.1016/j.jid.2023.06.191.
(In press).
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Hui, Gar Kay;
Gao, Xin;
Gor, Jayesh;
Lu, Jinghua;
Sun, Peter D;
Perkins, Stephen J;
(2023)
The solution structure of the unbound IgG Fc receptor CD64 resembles its crystal structure: Implications for function.
PloS One
, 18
(9)
, Article e0288351. 10.1371/journal.pone.0288351.
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I
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Iqbal, Hina;
Fung, Ka Wai;
Gor, Jayesh;
Bishop, Anthony C;
Makhatadze, George I;
Brodsky, Barbara;
Perkins, Stephen J;
(2023)
A solution structure analysis reveals a bent collagen triple helix in the complement activation recognition molecule mannan-binding lectin.
Journal of Biological Chemistry
, 299
(2)
, Article 102799. 10.1016/j.jbc.2022.102799.
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K
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Khalil, Mahmoud I;
Ismail, Heba M;
Panasyuk, Ganna;
Bdzhola, Anna;
Filonenko, Valeriy;
Gout, Ivan;
Pardo, Olivier E;
(2023)
Asymmetric Dimethylation of Ribosomal S6 Kinase 2 Regulates Its Cellular Localisation and Pro-Survival Function.
International Journal of Molecular Sciences
, 24
(10)
, Article 8806. 10.3390/ijms24108806.
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L
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Levray, Yvette S;
Bana, Bianca;
Tarr, Sarah J;
McLaughlin, Emilia J;
Rossi-Smith, Peter;
Waltho, Anita;
Charlton, Georgina H;
... Osborne, Andrew R; + view all
(2023)
Formation of ER-lumenal intermediates during export of Plasmodium proteins containing transmembrane-like hydrophobic sequences.
PLoS Pathogens
, 19
(3)
, Article e1011281. 10.1371/journal.ppat.1011281.
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M
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Meir, Amit;
Macé, Kévin;
Vegunta, Yogesh;
Williams, Sunanda M;
Waksman, Gabriel;
(2023)
Substrate recruitment mechanism by gram-negative type III, IV, and VI bacterial injectisomes.
Trends in Microbiology
10.1016/j.tim.2023.03.005.
(In press).
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Morfopoulou, Sofia;
Buddle, Sarah;
Montaguth, Oscar Enrique Torres;
Atkinson, Laura;
Guerra-Assunção, José Afonso;
Marjaneh, Mahdi Moradi;
Chiozzi, Riccardo Zenezini;
... Breuer, Judith; + view all
(2023)
Genomic investigations of unexplained acute hepatitis in children.
Nature
10.1038/s41586-023-06003-w.
(In press).
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Mühleip, Alexander;
Flygaard, Rasmus Kock;
Baradaran, Rozbeh;
Haapanen, Outi;
Gruhl, Thomas;
Tobiasson, Victor;
Maréchal, Amandine;
... Amunts, Alexey; + view all
(2023)
Structural basis of mitochondrial membrane bending by the I-II-III2-IV2 supercomplex.
Nature
10.1038/s41586-023-05817-y.
(In press).
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N
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Nallapareddy, Vamsi;
Bordin, Nicola;
Sillitoe, Ian;
Heinzinger, Michael;
Littmann, Maria;
Waman, Vaishali P;
Sen, Neeladri;
... Orengo, Christine; + view all
(2023)
CATHe: Detection of remote homologues for CATH superfamilies using embeddings from protein language models.
Bioinformatics
, 39
(1)
, Article btad029. 10.1093/bioinformatics/btad029.
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Newton, MD;
Losito, M;
Smith, QM;
Parnandi, N;
Taylor, BJ;
Akcakaya, P;
Maresca, M;
... Rueda, DS; + view all
(2023)
Negative DNA supercoiling induces genome-wide Cas9 off-target activity.
Molecular Cell
, 83
(19)
3533-3545.e5.
10.1016/j.molcel.2023.09.008.
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Ng, Joseph CF;
Montamat Garcia, Guillem;
Stewart, Alexander T;
Blair, Paul;
Mauri, Claudia;
Dunn-Walters, Deborah K;
Fraternali, Franca;
(2023)
sciCSR infers B cell state transition and predicts class-switch recombination dynamics using single-cell transcriptomic data.
Nature Methods
10.1038/s41592-023-02060-1.
(In press).
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Nicastro, Giuseppe;
Abis, Giancarlo;
Klein, Pierre;
Esteban-Serna, Sofia;
Gallagher, Christopher;
Chaves-Arquero, Belen;
Cai, Yuyang;
... Ramos, Andres; + view all
(2023)
Direct m6A recognition by IMP1 underlays an alternative model of target selection for non-canonical methyl-readers.
Nucleic Acids Research
, Article gkad534. 10.1093/nar/gkad534.
(In press).
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O
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O'Sullivan, James DB;
Blacker, Thomas S;
Scott, Claire;
Chang, Weise;
Ahmed, Mohi;
Yianni, Val;
Mann, Zoe F;
(2023)
Gradients of glucose metabolism regulate morphogen signalling required for specifying tonotopic organisation in the chicken cochlea.
Elife
, 12
, Article e86233. 10.7554/eLife.86233.
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Ofer, Sapir;
Blombach, Fabian;
Erkelens, Amanda M;
Barker, Declan;
Soloviev, Zoja;
Schwab, Samuel;
Smollett, Katherine;
... Werner, Finn; + view all
(2023)
DNA-bridging by an archaeal histone variant via a unique tetramerisation interface.
Communications Biology
, 6
(1)
, Article 968. 10.1038/s42003-023-05348-2.
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P
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Pálmadóttir, Tinna;
Waudby, Christopher A;
Bernfur, Katja;
Christodoulou, John;
Linse, Sara;
Malmendal, Anders;
(2023)
Morphology-Dependent Interactions between α-Synuclein Monomers and Fibrils.
International Journal of Molecular Sciences
, 24
(6)
, Article 5191. 10.3390/ijms24065191.
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Paysan-Lafosse, Typhaine;
Blum, Matthias;
Chuguransky, Sara;
Grego, Tiago;
Pinto, Beatriz Lázaro;
Salazar, Gustavo A;
Bileschi, Maxwell L;
... Bateman, Alex; + view all
(2023)
InterPro in 2022.
Nucleic Acids Research
, 51
(D1)
D418-D427.
10.1093/nar/gkac993.
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Petrone, Olivia;
Serafini, Steven;
Yu, Bess Yi Kun;
Filonenko, Valeriy;
Gout, Ivan;
O'Flaherty, Cristian;
(2023)
Changes of the Protein CoAlation Pattern in Response to Oxidative Stress and Capacitation in Human Spermatozoa.
International Journal of Molecular Sciences
, 24
(15)
, Article 12526. 10.3390/ijms241512526.
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Phillips, Ian R;
Veeravalli, Sunil;
Khadayate, Sanjay;
Shephard, Elizabeth A;
(2023)
Metabolomic and transcriptomic analyses of Fmo5-/- mice reveal roles for flavin-containing monooxygenase 5 (FMO5) in NRF2-mediated oxidative stress response, unfolded protein response, lipid homeostasis, and carbohydrate and one-carbon metabolism.
PLOS ONE
, 18
(6)
, Article e0286692. 10.1371/journal.pone.0286692.
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Plessa, Eleni;
(2023)
Studies of protein folding and misfolding on the ribosome.
Doctoral thesis (Ph.D), UCL (University College London).
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Poddar, Nilakhi;
Santini, Joanne M;
Maher, Megan J;
(2023)
The structure of the complex between the arsenite oxidase from Pseudorhizobium banfieldiae sp. strain NT-26 and its native electron acceptor cytochrome c552.
Acta Crystallographica Section D: Biological Crystallography
, D79
(4)
pp. 345-352.
10.1107/S2059798323002103.
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Prawiro, Christy;
Bunney, Tom D;
Kampyli, Charis;
Yaguchi, Hiroko;
Katan, Matilda;
Bangham, Charles RM;
(2023)
A frequent PLCγ1 mutation in adult T-cell leukemia/lymphoma determines functional properties of the malignant cells.
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
, 1869
(2)
, Article 166601. 10.1016/j.bbadis.2022.166601.
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R
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Rodríguez-López, María;
Bordin, Nicola;
Lees, Jon;
Scholes, Harry;
Hassan, Shaimaa;
Saintain, Quentin;
Kamrad, Stephan;
... Bähler, Jürg; + view all
(2023)
Broad functional profiling of fission yeast proteins using phenomics and machine learning.
eLife
, 12
10.7554/elife.88229.3.
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S
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Santini, Joanna M;
(2023)
Mechanisms of bioleaching: iron and sulfur oxidation by acidophilic microorganisms.
Essays in Biochemistry
, Article EBC20220257. 10.1042/EBC20220257.
(In press).
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Shukla, Vaibhav Kumar;
Heller, Gabriella T;
Hansen, D Flemming;
(2023)
Biomolecular NMR spectroscopy in the era of artificial intelligence.
Structure
10.1016/j.str.2023.09.011.
(In press).
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Shukla, Vaibhav Kumar;
Siemons, Lucas;
Hansen, D Flemming;
(2023)
Intrinsic structural dynamics dictate enzymatic activity and inhibition.
Proceedings of the National Academy of Sciences
, 120
(41)
, Article e2310910120. 10.1073/pnas.2310910120.
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Smith, Emily J;
Beaumont, Ross E;
McClellan, Alyce;
Sze, Cheryl;
Palomino Lago, Esther;
Hazelgrove, Liberty;
Dudhia, Jayesh;
... Guest, Deborah J; + view all
(2023)
Tumour necrosis factor alpha, interleukin 1 beta and interferon gamma have detrimental effects on equine tenocytes that cannot be rescued by IL-1RA or mesenchymal stromal cell–derived factors.
Cell and Tissue Research
, 391
(3)
pp. 523-544.
10.1007/s00441-022-03726-6.
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Soelistyo, Christopher;
Ulicna, Kristina;
Lowe, Alan;
(2023)
Machine learning enhanced cell tracking.
Frontiers in Bioinformatics
, 3
, Article 1228989. 10.3389/fbinf.2023.1228989.
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Soelistyo, Christopher Joseph;
(2023)
Learning biophysical determinants of cell fate in cell competition using interpretable deep neural networks.
Doctoral thesis (Ph.D), UCL (University College London).
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Soelistyo, CJ;
Charras, G;
Lowe, AR;
(2023)
Virtual perturbations to assess explainability of deep-learning based cell fate predictors.
In:
Proceedings of the 2023 IEEE/CVF International Conference on Computer Vision Workshops (ICCVW).
(pp. pp. 3973-3982).
IEEE: Paris, France.
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Stiens, Jennifer;
Tan, Yen Yi;
Joyce, Rosanna;
Arnvig, Kristine B;
Kendall, Sharon L;
Nobeli, Irene;
(2023)
Using a whole genome co-expression network to inform the functional characterisation of predicted genomic elements from Mycobacterium tuberculosis transcriptomic data.
Molecular Microbiology
, 119
(4)
pp. 381-400.
10.1111/mmi.15055.
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T
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Taunt, Henry N;
Jackson, Harry O;
Gunnarsson, Ísarr N;
Pervaiz, Rabbia;
Purton, Saul;
(2023)
Accelerating Chloroplast Engineering: A New System for Rapid Generation of Marker-Free Transplastomic Lines of Chlamydomonas reinhardtii.
Microorganisms
, 11
(8)
, Article 1967. 10.3390/microorganisms11081967.
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Tossounian, Maria-Armineh;
Baczynska, Maria;
Dalton, William;
Peak-Chew, Sew Yeu;
Undzenas, Kipras;
Korza, George;
Filonenko, Valeriy;
... Gout, Ivan; + view all
(2023)
Bacillus subtilis YtpP and Thioredoxin A Are New Players in the Coenzyme-A-Mediated Defense Mechanism against Cellular Stress.
Antioxidants
, 12
(4)
, Article 938. 10.3390/antiox12040938.
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Tossounian, Maria-Armineh;
Hristov, Stefan Denchev;
Semelak, Jonathan Alexis;
Yu, Bess Yi Kun;
Baczynska, Maria;
Zhao, Yuhan;
Estrin, Dario Ariel;
... Gout, Ivan; + view all
(2023)
A Unique Mode of Coenzyme A Binding to the Nucleotide Binding Pocket of Human Metastasis Suppressor NME1.
International Journal of Molecular Sciences
, 24
(11)
, Article 9359. 10.3390/ijms24119359.
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V
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Vallat, B;
Tauriello, G;
Bienert, S;
Haas, J;
Webb, BM;
Žídek, A;
Zheng, W;
... Westbrook, JD; + view all
(2023)
ModelCIF: An Extension of PDBx/mmCIF Data Representation for Computed Structure Models.
Journal of Molecular Biology
, Article 168021. 10.1016/j.jmb.2023.168021.
(In press).
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Van der Verren, Sander E;
Zanetti, Giulia;
(2023)
The small GTPase Sar1, control centre of COPII trafficking.
FEBS Letters
, 597
(6)
pp. 865-882.
10.1002/1873-3468.14595.
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Vancraenenbroeck, R;
Hofmann, H;
(2023)
Electrostatics and hydrophobicity in the dynamics of intrinsically disordered proteins.
European Physical Journal E
, 46
(12)
, Article 133. 10.1140/epje/s10189-023-00383-7.
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Vancraenenbroeck, Renée;
Cain, Amanda;
(2023)
Year 3 research project student-led inquiry labs with productive failure.
Presented at: Evolving Molecular Biosciences Education (Biochemical Society, FEBS, IUBMb), Manchester, UK.
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Varadi, M;
Bordin, N;
Orengo, C;
Velankar, S;
(2023)
The opportunities and challenges posed by the new generation of deep learning-based protein structure predictors.
Current Opinion in Structural Biology
, 79
, Article 102543. 10.1016/j.sbi.2023.102543.
(In press).
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Vickers, Sarah;
Irving, James;
Lomas, David A;
Thalassinos, Konstantinos;
(2023)
Native and Ion Mobility Mass Spectrometry Characterization of Alpha 1 Antitrypsin Variants and Oligomers.
In: Bristow, Cynthia L, (ed.)
Alpha-1 Antitrypsin: Methods and Protocols.
(pp. 41-55).
Humana Press (Springer): New York, NY, USA.
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Vilatte, Anaëlle;
Spencer-Milnes, Xenia;
Jackson, Harry Oliver;
Purton, Saul;
Parker, Brenda;
(2023)
Spray Drying Is a Viable Technology for the Preservation of Recombinant Proteins in Microalgae.
Microorganisms
, 11
(2)
, Article 512. 10.3390/microorganisms11020512.
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Vujkovic Bukvin, Ivana;
(2023)
Structures and targeting of the nascent polypeptide folding pathway on the ribosome.
Doctoral thesis (Ph.D), UCL (University College London).
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W
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Weston, TGR;
Rees, M;
Gautel, M;
Fraternali, F;
(2023)
Walking with giants: The challenges of variant impact assessment in the giant sarcomeric protein titin.
WIREs Mechanisms of Disease
, Article e1638. 10.1002/wsbm.1638.
(In press).
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Wikström, Mårten;
Gennis, Robert B;
Rich, Peter R;
(2023)
Structures of the intermediates in the catalytic cycle of mitochondrial cytochrome c oxidase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics
, 1864
(2)
, Article 148933. 10.1016/j.bbabio.2022.148933.
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X
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Xu, Ziqian;
Spencer, Holly J;
Harris, Victoria A;
Perkins, Stephen J;
(2023)
An updated interactive database for 1692 genetic variants in coagulation Factor IX provides detailed insights into haemophilia B.
Journal of Thrombosis and Haemostasis
, 21
(5)
pp. 1164-1176.
10.1016/j.jtha.2023.02.005.
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