Bdzhola, AV;
Filonenko, VV;
Gout, IT;
Malanchuk, OM;
(2023)
The use of the in situ proximity ligation assay for validating S6 kinase 1 CoAlation under oxidative stress.
Biopolymers & Cell
, 39
(4)
pp. 277-282.
10.7124/bc.000AA5.
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Abstract
Aim. To verify CoAlation of ribosomal protein S6 kinase 1 (S6K1) in the HEK293/Pank1b cells exposed to oxidative stress using proximity ligation assay (PLA). Methods. In situ proximi ty ligation assay. Results. S6 kinase 1 undergoes CoA covalent modification in cellular response to oxidative stress. Conclusions. The previously developed mass spectrometry-based methodology allowed us to identify over 2100 CoAlated proteins in the cells exposed to oxidative or metabolic stress. Many protein kinases were found to be CoAlated, including S6K1. In this study, the proximity ligation assay was used to demonstrate a significant increase of S6K1 CoAlation under oxidative stress compared to untreated cells.
| Type: | Article |
|---|---|
| Title: | The use of the in situ proximity ligation assay for validating S6 kinase 1 CoAlation under oxidative stress |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.7124/bc.000AA5 |
| Publisher version: | http://dx.doi.org/10.7124/bc.000aa5 |
| Language: | English |
| Additional information: | © Publisher PH "Akademperiodyka" of the NAS of Ukraine, 2023 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited |
| Keywords: | CoA, S6 kinase 1, in situ proximity ligation assay, post-translation modification, oxidative stress, signal transduction, confocal microscopy, cell growth, antibodies. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10190570 |
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