Tansirichaiya, S;
Rahman, MA;
Antepowicz, A;
Mullany, P;
Roberts, AP;
(2016)
Detection of Novel Integrons in the Metagenome of Human Saliva.
PLOS ONE
, 11
(6)
, Article e0157605. 10.1371/journal.pone.0157605.
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Abstract
Integrons are genetic elements capable of capturing and expressing open reading frames (ORFs) embedded within gene cassettes. They are involved in the dissemination of antibiotic resistance genes (ARGs) in clinically important pathogens. Although the ARGs are common in the oral cavity the association of integrons and antibiotic resistance has not been reported there. In this work, a PCR-based approach was used to investigate the presence of integrons and associated gene cassettes in human oral metagenomic DNA obtained from both the UK and Bangladesh. We identified a diverse array of gene cassettes containing ORFs predicted to confer antimicrobial resistance and other adaptive traits. The predicted proteins include a putative streptogramin A O-acetyltransferase, a bleomycin binding protein, cof-like hydrolase, competence and motility related proteins. This is the first study detecting integron gene cassettes directly from oral metagenomic DNA samples. The predicted proteins are likely to carry out a multitude of functions; however, the function of the majority is yet unknown.
| Type: | Article |
|---|---|
| Title: | Detection of Novel Integrons in the Metagenome of Human Saliva |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1371/journal.pone.0157605 |
| Publisher version: | http://dx.doi.org/10.1371/journal.pone.0157605 |
| Language: | English |
| Additional information: | Copyright: © 2016 Tansirichaiya et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Keywords: | Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, MOBILE GENE CASSETTES, TOXIN-ANTITOXIN LOCI, ANTIBIOTIC-RESISTANCE, BACTERIAL EVOLUTION, ENVIRONMENTAL DNA, RNA, RECOMBINATION, RECOVERY, SEARCH, MICROBIOTA |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Microbial Diseases |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1501647 |
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