Tulay, P;
Naja, RP;
Cascales-Roman, O;
Doshi, A;
Serhal, P;
SenGupta, SB;
(2015)
Investigation of microRNA expression and DNA repair gene transcripts in human oocytes and blastocysts.
Journal of Assisted Reproduction and Genetics
, 32
(12)
pp. 1757-1764.
10.1007/s10815-015-0585-0.
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Abstract
PURPOSE: The aim of the study is to investigate the regulation of DNA repair genes by microRNAs (miRNAs). miRNAs are short non-coding RNAs that regulate transcriptional and post-transcriptional gene silencing. Several miRNAs that are expressed during preimplantation embryo development have been shown or are predicted to target genes that regulate cell cycle checkpoints and DNA repair in response to DNA damage. METHODS: This study compares the expression level of 20 miRNAs and 9 target transcripts involved in DNA repair. The statistical significance of differential miRNA expression between oocytes and blastocysts was determined by t test analysis using the GraphPad Prism v6 software. The possible regulatory roles of miRNAs on their target messenger RNAs (mRNAs) were analysed using a Pearson correlation test. RESULTS: This study shows for the first time that several miRNAs are expressed in human oocytes and blastocysts that target key genes involved in DNA repair and cell cycle checkpoints. Blastocysts exhibited statistically significant lower expression levels for the majority of miRNAs compared to oocytes (p < 0.05). Correlation analyses showed that there was both inverse and direct association between miRNAs and their target mRNAs. CONCLUSIONS: miRNAs target many mRNAs including ones involved in DNA repair mechanisms. This study suggests that miRNAs and their target mRNAs involved in DNA repair are expressed in preimplantation embryos. Similar to the miRNAs expressed in adult tissues, these miRNAs seem to have regulatory roles on their target DNA repair mRNAs during preimplantation embryo development.
| Type: | Article |
|---|---|
| Title: | Investigation of microRNA expression and DNA repair gene transcripts in human oocytes and blastocysts |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s10815-015-0585-0 |
| Publisher version: | http://doi.org/10.1007/s10815-015-0585-0 |
| Language: | English |
| Additional information: | © Springer Science+Business Media New York 2015. The final publication is available at Springer via http://dx.doi.org/10.1007/s10815-015-0585-0 |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Genetics & Heredity, Obstetrics & Gynecology, Reproductive Biology, MicroRNA, Expression, DNA repair, Human oocyte and blastocyst, Preimplantation embryo development, EARLY EMBRYONIC-DEVELOPMENT, MISMATCH REPAIR, MESSENGER-RNAS, BREAST-CANCER, NONCODING RNA, IDENTIFICATION, TARGETS, CELLS, RECOGNITION, BIOGENESIS |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health > Reproductive Health |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1483616 |
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