Garbo, Sabrina;
D'Andrea, Daniel;
Colantoni, Alessio;
Fiorentino, Francesco;
Mai, Antonello;
Ramos, Andres;
Tartaglia, Gian Gaetano;
... Battistelli, Cecilia; + view all
(2024)
m6A modification inhibits miRNAs’ intracellular function, favoring their extracellular export for intercellular communication.
Cell Reports
, 43
(6)
, Article 114369. 10.1016/j.celrep.2024.114369.
Preview |
PDF
1-s2.0-S2211124724006971-mmc1.pdf - Published Version Download (606kB) | Preview |
Preview |
PDF
1-s2.0-S2211124724006971-main.pdf - Published Version Download (4MB) | Preview |
Abstract
Epitranscriptomics represents a further layer of gene expression regulation. Specifically, N6-methyladenosine (m6A) regulates RNA maturation, stability, degradation, and translation. Regarding microRNAs (miRNAs), while it has been reported that m6A impacts their biogenesis, the functional effects on mature miRNAs remain unclear. Here, we show that m6A modification on specific miRNAs weakens their coupling to AGO2, impairs their function on target mRNAs, determines their delivery into extracellular vesicles (EVs), and provides functional information to receiving cells. Mechanistically, the intracellular functional impairment is caused by m6A-mediated inhibition of AGO2/miRNA interaction, the EV loading is favored by m6A-mediated recognition by the RNA-binding protein (RBP) hnRNPA2B1, and the EV-miRNA function in the receiving cell requires their FTO-mediated demethylation. Consequently, cells express specific miRNAs that do not impact endogenous transcripts but provide regulatory information for cell-to-cell communication. This highlights that a further level of complexity should be considered when relating cellular dynamics to specific miRNAs.
| Type: | Article |
|---|---|
| Title: | m6A modification inhibits miRNAs’ intracellular function, favoring their extracellular export for intercellular communication |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.celrep.2024.114369 |
| Publisher version: | https://doi.org/10.1016/j.celrep.2024.114369 |
| Language: | English |
| Additional information: | © 2024 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/). |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Cell Biology, MESSENGER-RNA, M(6)A MODIFICATION, STRUCTURAL BASIS, N-6-METHYLADENOSINE, EPITRANSCRIPTOME |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10210124 |
Archive Staff Only
 |
View Item |
