Tortolini, C;
Gigli, V;
Angeloni, A;
Tasca, F;
Thanh, NTK;
Antiochia, R;
(2024)
A disposable immunosensor for the detection of salivary MMP-8 as biomarker of periodontitis.
Bioelectrochemistry
, 156
, Article 108590. 10.1016/j.bioelechem.2023.108590.
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Abstract
This work describes the development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care. The electrochemical platform was based on a graphene (GPH) screen-printed electrode (SPE) functionalized by gold-nanospheres (AuNSs) and antibodies against MMP-8 protein (anti-MMP-8). The functionalization with anti-MMP-8 was realized by using 11-mercaptoundecanoic acid (11-MUA), thanks to its ability to give strong sulfur bonds with its –SH end, and to cross-link the –NH2 groups of the antibody molecule with the other –COOH end, using the traditional EDC-NHS method. The voltammetric sensor showed good performances with a linear range of 2.5–300 ng mL−1, a LOD value of 1.0 ± 0.1 ng mL−1 and a sensitivity of 0.05 µA mL cm−2 ng−1. Moreover, the proposed immunosensor was tested in real saliva samples, showing comparable results to those obtained with the conventional ELISA method. The biosensor was single-use and cost-effective and required a small quantity of test medium and a short preparation time, representing a very attractive biosensor for MMP-8 detection in human saliva.
| Type: | Article |
|---|---|
| Title: | A disposable immunosensor for the detection of salivary MMP-8 as biomarker of periodontitis |
| Location: | Netherlands |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.bioelechem.2023.108590 |
| Publisher version: | https://doi.org/10.1016/j.bioelechem.2023.108590 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10190334 |
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