Yusuf, M;
Farooq, S;
Robinson, I;
Lalani, EN;
(2020)
Cryo-nanoscale chromosome imaging—future prospects.
Biophysical Reviews
, 12
pp. 1257-1263.
10.1007/s12551-020-00757-7.
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Abstract
The high-order structure of mitotic chromosomes remains to be fully elucidated. How nucleosomes compact at various structural levels into a condensed mitotic chromosome is unclear. Cryogenic preservation and imaging have been applied for over three decades, keeping biological structures close to the native in vivo state. Despite being extensively utilized, this field is still wide open for mitotic chromosome research. In this review, we focus specifically on cryogenic efforts for determining the mitotic nanoscale chromatin structures. We describe vitrification methods, current status, and applications of advanced cryo-microscopy including future tools required for resolving the native architecture of these fascinating structures that hold the instructions to life.
| Type: | Article |
|---|---|
| Title: | Cryo-nanoscale chromosome imaging—future prospects |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s12551-020-00757-7 |
| Publisher version: | https://doi.org/10.1007/s12551-020-00757-7 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | mitotic, chromosome, chromatin, nanoscale, cryo, freezing, microscopy |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10112038 |
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