Browse by UCL people
Group by: Type | Date
Number of items: 43.
2025
Chen, Qiantong;
Lee, Chae Hyon;
Whitfield, Robert;
Nesbeth, Darren N;
(2025)
Chronologically distributed transfection improves AAV2 and AAV2/8 capsid filling and reveals assembly schedule divergence.
Molecular Therapy: Methods and Clinical Development
, Article 101610. 10.1016/j.omtm.2025.101610.
(In press).
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Elgabry, Mariam;
Nesbeth, Darren;
Ekblom, Paul;
Johnson, Shane;
(2025)
BAKE: a novel framework for iterative security design for identifying criminally-exploitable vulnerabilities in biotechnology products.
Crime Science
, 14
, Article 16. 10.1186/s40163-025-00256-8.
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Jonsson, KG;
Nesbeth, D;
Keshavarz-Moore, E;
(2025)
Novel approaches to engineering autolysis: A scalable alternative for recovery of bioproducts.
Journal of Biotechnology
, 406
pp. 115-126.
10.1016/j.jbiotec.2025.07.007.
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Mazlan, Farhaneen A;
Wasmuth, Matthew AJ;
Nesbeth-Bain, Mahalia EM;
Nesbeth, Darren N;
(2025)
Halal considerations that signpost a cellular agriculture compatible with world religions.
Trends in Food Science & Technology
, 161
, Article 105015. 10.1016/j.tifs.2025.105015.
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2024
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Howe, Geoffrey;
Bal, Mehtap;
Wasmuth, Matthew;
Massaro, G;
Rahim, Ahad;
Ali, Sadfer;
Rivera, Milena;
... Nesbeth, Darren; + view all
(2024)
An autonucleolytic suspension HEK293F host cell line for high-titre serum-free AAV5 and AAV9 production with reduced levels of DNA impurity.
Molecular Therapy - Methods and Clinical Development
, Article 101317. 10.1016/j.omtm.2024.101317.
(In press).
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Howe, Geoffrey;
Wasmuth, matthew;
Emanuelle, Pamela;
Massaro, Giulia;
Rahim, Ahad;
Ali, sadfer;
Rivera, Milena;
... Nesbeth, Darren; + view all
(2024)
Engineering an autonucleolytic mammalian suspension host cell line to reduce DNA impurity levels in serum-free lentiviral process streams.
ACS Synthetic Biology
, 13
(2)
pp. 408-692.
10.1021/acssynbio.3c00682.
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Redwood-Sawyerr, Chileab;
Howe, Geoffrey;
Theodore, Andalucia Evans;
Nesbeth, Darren N;
(2024)
Genetically Encoded Trensor Circuits Report HeLa Cell Treatment with Polyplexed Plasmid DNA and Small-Molecule Transfection Modulators.
ACS Synthetic Biology
, 13
(10)
pp. 3163-3172.
10.1021/acssynbio.4c00148.
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2023
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Ali, Sadfer;
Rivera, Milena;
Ward, John;
Keshavarz-Moore, Eli;
Mason, Chris;
Nesbeth, Darren;
(2023)
Serum-free lentiviral vector production is compatible with medium-resident nuclease activity arising from adherent HEK293T host cells engineered with a nuclease-encoding transgene.
Heliyon
, 9
(6)
, Article e17067. 10.1016/j.heliyon.2023.e17067.
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Bandyopadhyay, Sushobhan;
Pavlika, Vasos;
Bracewell, Daniel;
Nesbeth, Darren;
(2023)
A biological OR(XNOR) logic gate couples carbon source and transgene expression switching in a Komagatella phaffii (Pichia pastoris) strain co-producing process-enhancing lipase and a virus-like particle (VLP) vaccine.
ACS Synthetic Biology
, 12
(3)
pp. 657-663.
10.1021/acssynbio.2c00342.
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Redwood-Sawyerr, Chileab;
Nesbeth, Darren N;
(2023)
Tetracycline-mediated fluorescence correlates with passage number and β-galactosidase activity in HeLa and HEK293T cells.
Biology Open
, 12
(7)
, Article bio059691. 10.1242/bio.059691.
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2022
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Borg, Yanika;
Alsford, Sam;
Pavlika, Vasos;
Zaikin, Alexey;
Nesbeth, Darren;
(2022)
Synthetic biology tools for engineering Goodwin oscillation in Trypanosoma brucei brucei.
Heliyon
, 8
(2)
, Article e08891. 10.1016/j.heliyon.2022.e08891.
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Elgabry, Mariam;
Nesbeth, Darren;
Johnson, Shane;
(2022)
The Future of Biotechnology Crime: A Parallel Delphi Study with Non-Traditional Experts.
Futures
, 141
, Article 102970. 10.1016/j.futures.2022.102970.
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Kriston-Vizi, Janos;
Lenart, Izabela;
Iwawaki, Takao;
Gould, Keith;
Nesbeth, Darren;
Powis, Simon J;
Antoniou, Antony N;
(2022)
Salmonella Exhibit Altered Cellular Localization in the Presence of HLA-B27 and Codistribute with Endo-Reticular Membrane.
Journal of Immunology Research
, 2022
, Article 9493019. 10.1155/2022/9493019.
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Lenart, Isabella;
Truong, L-H;
Nguyen, D;
Kumar, P;
McHugh, K;
Pereira, B;
Maan, B;
... Antoniou, Anthony; + view all
(2022)
Intrinsic Folding Properties of the HLA-B27 Heavy Chain Revealed by Single Chain Trimer Versions of Peptide-Loaded Class I Major Histocompatibility Complex Molecules.
Frontiers in Immunology
, 13
, Article 902135. 10.3389/fimmu.2022.902135.
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Panzella, L;
Benning, K;
Nesbeth, DN;
Setaro, B;
D'Errico, G;
Napolitano, A;
d'Ischia, M;
(2022)
Identification of black sturgeon caviar pigment as eumelanin.
Food Chemistry
, 373
(B)
, Article 131474. 10.1016/j.foodchem.2021.131474.
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2021
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Bandyopadhyay, S;
Morris, SA;
Nesbeth, DN;
Bracewell, DG;
(2021)
Lipid reduction to improve clarification and filterability during primary recovery of intracellular products in yeast lysates using exogenous lipase.
Journal of Chemical Technology & Biotechnology
, 96
(11)
pp. 3166-3174.
10.1002/jctb.6871.
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2020
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Elgabry, M;
Nesbeth, D;
Johnson, SD;
(2020)
A Systematic Review of the Criminogenic Potential of Synthetic Biology and Routes to Future Crime Prevention.
Frontiers in Bioengineering and Biotechnology
, 8
, Article 571672. 10.3389/fbioe.2020.571672.
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Elgabry, M;
Nesbeth, D;
Johnson, SD;
(2020)
A systematic review protocol for crime trends facilitated by synthetic biology.
Systematic Reviews
, 9
, Article 22. 10.1186/s13643-020-1284-1.
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Henríquez, M-J;
Braun-Galleani, S;
Nesbeth, D;
(2020)
Whole cell biosynthetic activity of Komagataella phaffii (Pichia pastoris) GS115 strains engineered with transgenes encoding Chromobacterium violaceum ω-transaminase alone or combined with native transketolase.
Biotechnology Progress
, 36
(1)
, Article e2893. 10.1002/btpr.2893.
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Henríquez, MJ;
Cardós-Elena, RP;
Nesbeth, D;
(2020)
Genomic data mining reveals the transaminase repertoire of Komagataella phaffii (Pichia pastoris) strain GS115 and supports a systematic nomenclature.
Journal of Genetics
, 99
, Article 49. 10.1007/s12041-020-01201-1.
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2019
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Antoniou, A;
Lenart, I;
Kriston-Vizi, J;
Takao, I;
Turmaine, M;
McHugh, K;
Ali, S;
... Powis, S; + view all
(2019)
Salmonella exploits HLA-B27 and host Unfolded Protein Responses to promote intracellular replication.
Annals of the Rheumatic Diseases
, 78
(1)
pp. 74-82.
10.1136/ annrheumdis-2018-213532.
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Braun-Galleani, S;
Henríquez, M-J;
Nesbeth, D;
(2019)
Whole cell biosynthesis of 1-methyl-3-phenylpropylamine and 2-amino-1,3,4-butanetriol using Komagataella phaffii (Pichia pastoris) strain BG-10 engineered with a transgene encoding Chromobacterium violaceum ω- transaminase.
Heliyon
, 5
(8)
, Article e02338. 10.1016/j.heliyon.2019.e02338.
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Folarin, O;
Nesbeth, D;
Ward, JM;
Keshavarz-Moore, E;
(2019)
Application of Plasmid Engineering to Enhance Yield and Quality of Plasmid for Vaccine and Gene Therapy.
Bioengineering
, 6
(2)
, Article 54. 10.3390/bioengineering6020054.
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Santiago Toledo, Gerardo;
(2019)
Development of a xeno-free cell culture platform for novel candidate human cell therapies for neural regeneration.
Doctoral thesis (Ph.D), UCL (University College London).
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2018
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Boeing, P;
Leon, M;
Nesbeth, DN;
Finkelstein, A;
Barnes, C;
(2018)
Towards an Aspect-Oriented Design and Modelling Framework for Synthetic Biology.
Processes
, 6
(9)
, Article 167. 10.3390/pr6090167.
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Wei, Y-C;
Braun-Galleani, S;
Henriquez, M-J;
Bandara, S;
Nesbeth, DN;
(2018)
Biotransformation of β-Hydroxypyruvate and Glycolaldehyde to L-Erythrulose by Pichia pastoris strain GS115 overexpressing native Transketolase.
Biotechnology Progress
, 34
(1)
pp. 99-106.
10.1002/btpr.2577.
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2017
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Kinna, AW;
(2017)
Improved production and purification of recombinant proteins from mammalian expression systems.
Doctoral thesis , UCL (University College London).
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Liu, S;
Ruban, L;
Zhou, Y;
Wang, Y;
Nesbeth, DN;
(2017)
Establishing elements of a synthetic biology platform for Vaccinia virus production: BioBrickTM design, serum-free virus production and microcarrier-based cultivation of CV-1 cells.
Heliyon
, 3
(2)
, Article e00238. 10.1016/j.heliyon.2017.e00238.
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Schofield, DM;
Nesbeth, DN;
(2017)
A high performance bench scale process for isolation from inclusion bodies, refolding and dimerisation of a thiol-engineered recombinant therapeutic protein.
Biotechnology and Bioprocess Engineering
, 22
(4)
pp. 423-430.
10.1007/s12257-016-0385-0.
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Schofield, DM;
Sirka, E;
Keshavarz-Moore, E;
Ward, JM;
Nesbeth, DN;
(2017)
Improving Fab’ fragment retention in an autonucleolytic Escherichia coli strain by swapping periplasmic nuclease translocation signal from OmpA to DsbA.
Biotechnology Letters
, 39
(12)
pp. 1865-1873.
10.1007/s10529-017-2425-z.
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Templar, A;
(2017)
Industrially Robust Synthetic Biology Standards for the Polymerase Chain Reaction.
Doctoral thesis , UCL (University College London).
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Templar, A;
Schofield, DM;
Nesbeth, DN;
(2017)
Measuring E. coli and bacteriophage DNA in cell sonicates to evaluate the CAL1 reaction as a synthetic biology standard for qPCR.
Biomolecular Detection and Quantification
, 11
pp. 21-30.
10.1016/j.bdq.2016.12.001.
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2016
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Borg, Y;
Grigonyte, AM;
Boeing, P;
Wolfenden, B;
Smith, P;
Beaufoy, W;
Rose, S;
... Nesbeth, DN; + view all
(2016)
Open source approaches to establishing Roseobacter Glade bacteria as synthetic biology chassis for biogeoengineering.
PeerJ
, 4
, Article e2031. 10.7717/peerj.2031.
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Kinna, A;
Tolner, B;
Rota, EM;
Titchener-Hooker, N;
Nesbeth, D;
Chester, K;
(2016)
IMAC capture of recombinant protein from unclarified mammalian cell feed streams.
Biotechnology and Bioengineering
, 113
(1)
pp. 130-140.
10.1002/bit.25705.
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Liu, S;
Ruban, L;
Wang, Y;
Zhou, Y;
Nesbeth, DN;
(2016)
Establishing elements of a synthetic biology platform for Vaccinia virus production: BioBrickTM design, serum-free virus production and microcarrier-based cultivation of CV-1 cells.
BioRxiv
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Nesbeth, DN;
Zaikin, A;
Saka, Y;
Romano, MC;
Giuraniuc, CV;
Kanakov, O;
Laptyeva, T;
(2016)
Synthetic biology routes to bio-artificial intelligence.
Essays in Biochemistry
, 60
(4)
pp. 381-391.
10.1042/EBC20160014.
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Schofield, DM;
(2016)
Synthetic biology approaches to bioprocess intensification in bacterial and mammalian production platforms.
Doctoral thesis , UCL (University College London).
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Schofield, DM;
Templar, A;
Newton, J;
Nesbeth, DN;
(2016)
Promoter engineering to optimise recombinant periplasmic Fab' fragment production in Escherichia coli.
Biotechnology Progress
, 32
(4)
pp. 840-847.
10.1002/btpr.2273.
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Templar, A;
Marsh, D;
Nesbeth, DN;
(2016)
A synthetic biology standard for Chinese Hamster Ovary cell genome monitoring and contaminant detection by polymerase chain reaction.
SpringerPlus
, 5
, Article 1510. 10.1186/s40064-016-3074-8.
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Templar, A;
Woodhouse, S;
Keshavarz-Moore, E;
Nesbeth, DN;
(2016)
Influence of Pichia pastoris cellular material on polymerase chain reaction performance as a synthetic biology standard for genome monitoring.
Journal of Microbiological Methods
, 127
pp. 111-122.
10.1016/j.mimet.2016.05.013.
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2015
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Borg, Y;
(2015)
Design, Mathematical Modelling, Construction and Testing of Synthetic Gene Network Oscillators to Establish Roseobacter Clade Bacteria and the Protozoan Trypanosoma brucei as Synthetic Biology Chassis.
Doctoral thesis , UCL (University College London).
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2014
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Borg, Y;
Ullner, E;
Alagha, A;
Alsaedi, A;
Nesbeth, D;
Zaikin, A;
(2014)
Complex and unexpected dynamics in simple genetic regulatory networks.
INTERNATIONAL JOURNAL OF MODERN PHYSICS B
, 28
(14)
, Article ARTN 1430006. 10.1142/S0217979214300060.
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