Thompson, H;
Camand, O;
Barker, D;
Erskine, L;
(2006)
Slit proteins regulate distinct aspects of retinal ganglion cell axon guidance within dorsal and ventral retina.
Journal of Neuroscience
, 26
(31)
8082 - 8091.
10.1523/JNEUROSCI.1342-06.2006.
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Abstract
An early step in the formation of the optic pathway is the directed extension of retinal ganglion cell (RGC) axons into the optic fiber layer (OFL) of the retina in which they project toward the optic disc. Using analysis of knock-out mice and in vitro assays, we found that, in the mammalian retina, Slit1 and Slit2, known chemorepellents for RGC axons, regulate distinct aspects of intraretinal pathfinding in different regions of the retina. In ventral and, to a much lesser extent, dorsal retina, Slits help restrict RGC axons to the OFL. Additionally, within dorsal retina exclusively, Slit2 also regulates the initial polarity of outgrowth from recently differentiated RGCs located in the retinal periphery. This regional specificity occurs despite the fact that Slits are expressed throughout the retina, and both dorsal and ventral RGCs are responsive to Slits. The gross morphology and layering of the retina of the slit-deficient retinas is normal, demonstrating that these distinct guidance defects are not the result of changes in the organization of the tissue. Although displaced or disorganized, the aberrant axons within both dorsal and ventral retina exit the eye. We also have found that the lens, which because of its peripheral location within the developing eye is ideally located to influence the initial direction of RGC axon outgrowth, secretes Slit2, suggesting this is the source of Slit regulating OFL development. These data demonstrate clearly that multiple mechanisms exist in the retina for axon guidance of which Slits are an important component
| Type: | Article |
|---|---|
| Title: | Slit proteins regulate distinct aspects of retinal ganglion cell axon guidance within dorsal and ventral retina |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1523/JNEUROSCI.1342-06.2006 |
| Language: | English |
| Additional information: | Aug 2Slit proteins regulate distinct aspects of retinal ganglion cell axon guidance within dorsal and ventral retina16885222065606/Wellcome Trust 072495/Wellcome Trust Journal Article Research Support, Non-U.S. Gov\'t United States the official journal of the Society for Neuroscienceeng This work is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The license allows you to copy, distribute, and transmit the work, as well as adapting it. However, you must attribute the work to the author (but not in any way that suggests that they endorse you or your use of the work), and cannot use the work for commercial purposes without prior permission of the author. If you alter or build upon this work, you can distribute the resulting work only under the same or similar license to this one. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ or send a letter to Creative Commons, 444 Castro Street, Suite 900, Mountain View, California, 94041, USA. |
| Keywords: | Adaptation, Physiological/physiology Animals Axons/*physiology/*ultrastructure Body Patterning/*physiology Cells, Cultured Gene Expression Regulation, Developmental/physiology Mice Mice, Knockout Nerve Tissue Proteins/*metabolism Retina/*embryology/metabo |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/183338 |
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