O'Rourke, C;
Lee-Reeves, C;
Drake, RA;
Cameron, GW;
Loughlin, AJ;
Phillips, JB;
(2017)
Adapting tissue-engineered in vitro CNS models for high-throughput study of neurodegeneration.
Journal of Tissue Engineering
, 8
pp. 1-9.
10.1177/2041731417697920.
Preview |
Text
Adapting tissue-engineered in vitro CNS models for high-throughput study of neurodegeneration.pdf - Published Version Download (753kB) | Preview |
Abstract
Neurodegenerative conditions remain difficult to treat, with the continuing failure to see therapeutic research successfully advance to clinical trials. One of the obstacles that must be overcome is to develop enhanced models of disease. Tissue engineering techniques enable us to create organised artificial central nervous system tissue that has the potential to improve the drug development process. This study presents a replicable model of neurodegenerative pathology through the use of engineered neural tissue co-cultures that can incorporate cells from various sources and allow degeneration and protection of neurons to be observed easily and measured, following exposure to neurotoxic compounds - okadaic acid and 1-methyl-4-phenylpyridinium. Furthermore, the technology has been miniaturised through development of a mould with 6 mm length that recreates the advantageous features of engineered neural tissue co-cultures at a scale suitable for commercial research and development. Integration of human-derived induced pluripotent stem cells aids more accurate modelling of human diseases, creating new possibilities for engineered neural tissue co-cultures and their use in drug screening.
| Type: | Article |
|---|---|
| Title: | Adapting tissue-engineered in vitro CNS models for high-throughput study of neurodegeneration |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1177/2041731417697920 |
| Publisher version: | http://dx.doi.org/10.1177/2041731417697920 |
| Language: | English |
| Additional information: | This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
| Keywords: | Neurodegeneration, drug screening, induced pluripotent stem cells, neurons, three-dimensional models |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmacology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1557810 |
Loading...
Loading...Loading...Loading...Archive Staff Only
 |
View Item |
