Van Lessen, M;
Shibata-Germanos, S;
Van Impel, A;
Hawkins, TA;
Rihel, J;
Schulte-Merker, S;
(2017)
Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development.
eLife
, 2017
(6)
, Article e25932. 10.7554/eLife.25932.
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Abstract
The lymphatic system controls fluid homeostasis and the clearance of macromolecules from interstitial compartments. In mammals brain lymphatics were only recently discovered, with significant implications for physiology and disease. We examined zebrafish for the presence of brain lymphatics and found loosely connected endothelial cells with lymphatic molecular signature covering parts of the brain without forming endothelial tubular structures. These brain lymphatic endothelial cells (BLECs) derive from venous endothelium, are distinct from macrophages, and are sensitive to loss of Vegfc. BLECs endocytose macromolecules in a selective manner, which can be blocked by injection of mannose receptor ligands. This first report on brain lymphatic endothelial cells in a vertebrate embryo identifies cells with unique features, including the uptake of macromolecules at a single cell level. Future studies will address whether this represents an uptake mechanism that is conserved in mammals and how these cells affect functions of the embryonic and adult brain.
Type: | Article |
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Title: | Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development |
Location: | England |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.7554/eLife.25932 |
Publisher version: | http://dx.doi.org/10.7554/eLife.25932 |
Language: | English |
Additional information: | Copyright © van Lessen et al. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use and redistribution provided that the original author and source are credited. |
Keywords: | developmental biology, stem cells, zebrafish |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
URI: | https://discovery.ucl.ac.uk/id/eprint/1556373 |
1. | United States | 7 |
2. | China | 3 |
3. | Egypt | 1 |
4. | Indonesia | 1 |
5. | United Kingdom | 1 |
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