Audzevich, T; Bashford-Rogers, R; Mabbott, NA; Frampton, D; Freeman, TC; Potocnik, A; Kellam, P; Audzevich, T; Bashford-Rogers, R; Mabbott, NA; Frampton, D; Freeman, TC; Potocnik, A; Kellam, P; Gilroy, DW; - view fewer (2017) Pre/pro-B cells generate macrophage populations during homeostasis and inflammation. Proceedings of The National Academy of Sciences of The United States of America (PNAS) , 114 (20) E3954-E3963. 10.1073/pnas.1616417114.

Preview	Text Gilroy-D_pro-B cells generate macrophage populations_Manuscript.pdf - Accepted Version Available under License : See the attached licence file. Download (531kB) | Preview
	Text 392021_2_other__hmxhkk.pdf - Accepted Version Available under License : See the attached licence file. Download (3MB)
	Slideshow Gilroy_pro-B cells generate macrophage populations__Figure 1 (re-formatted).pptx - Accepted Version Available under License : See the attached licence file. Download (322kB)
	Slideshow Gilroy-D_pro-B cells generate macrophage populations__Figure 2 (re-formatted).pptx - Accepted Version Available under License : See the attached licence file. Download (13MB)
	Slideshow Gilroy-D_pro-B cells generate macrophage populations__Figure 3.pptx - Accepted Version Available under License : See the attached licence file. Download (643kB)
	Slideshow Gilroy-D_pro-B cells generate macrophage populations__Figure 4.pptx - Accepted Version Available under License : See the attached licence file. Download (516kB)
	Slideshow Gilroy-D_pro-B cells generate macrophage populations__Figure 5.pptx - Accepted Version Available under License : See the attached licence file. Download (1MB)
	Slideshow Gilroy-D_pro-B cells generate macrophage populations__Figure 6 (re-formatted) B.pptx - Accepted Version Available under License : See the attached licence file. Download (2MB)
	Slideshow Gilroy-D_pro-B cells generate macrophage populations__Figure 7.pptx - Accepted Version Available under License : See the attached licence file. Download (633kB)
	Slideshow Gilroy-D_pro-B cells generate macrophage populations__Figure 8.pptx - Accepted Version Available under License : See the attached licence file. Download (1MB)
Preview	Text Gilroy-D_pro-B cells generate macrophage populations__Supplementary data_Tables.pdf - Accepted Version Available under License : See the attached licence file. Download (8MB) | Preview

Abstract

Most tissue-resident macrophages (Mφs) are believed to be derived prenatally and are assumed to maintain themselves throughout life by self-proliferation. However, in adult mice we identified a progenitor within bone marrow, early pro-B cell/fraction B, that differentiates into tissue Mφs. These Mφ precursors have non-rearranged B-cell receptor genes and coexpress myeloid (GR1, CD11b, and CD16/32) and lymphoid (B220 and CD19) lineage markers. During steady state, these precursors exit bone marrow, losing Gr1, and enter the systemic circulation, seeding the gastrointestinal system as well as pleural and peritoneal cavities but not the brain. While in these tissues, they acquire a transcriptome identical to embryonically derived tissue-resident Mφs. Similarly, these Mφ precursors also enter sites of inflammation, gaining CD115, F4/80, and CD16/32, and become indistinguishable from blood monocyte-derived Mφs. Thus, we have identified a population of cells within the bone marrow early pro-B cell compartment that possess functional plasticity to differentiate into either tissue-resident or inflammatory Mφs, depending on microenvironmental signals. We propose that these precursors represent an additional source of Mφ populations in adult mice during steady state and inflammation.

Download activity - last month

Export as CSVXMLJSON

Download activity - last 12 months

Export as CSVXMLJSON

Downloads by country - last 12 months

Export as CSVXMLJSON

Archive Staff Only

View Item