Ferrara, G;
Valentini, D;
Rao, M;
Wahlstrom, J;
Grunewald, J;
Larsson, L-O;
Brighenti, S;
... Maeurer, M; + view all
(2017)
Humoral immune profiling of mycobacterial antigen recognition in sarcoidosis and Lofgren's syndrome using high-content peptide microarrays.
International Journal of Infectious Diseases
, 56
pp. 167-175.
10.1016/j.ijid.2017.01.021.
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Abstract
INTRODUCTION: Sarcoidosis is considered an idiopathic granulomatous disease, although similar immunological and clinical features with tuberculosis (TB) suggest mycobacterial involvement in its pathogenesis. High-content peptide microarrays (HCPM) may help to decipher mycobacteria-specific antibody reactivity in sarcoidosis. METHODS: Serum samples from patients with sarcoidosis, Löfgren’s syndrome, and TB, as well as from healthy individuals (12/group), were tested on HCPM containing 5964 individual peptides spanning 154 Mycobacterium tuberculosis proteins displayed as 15-amino acid stretches. Inclusion/exclusion and significance analyses were performed according to published methods. RESULTS: Each study group recognized 68–78% M. tuberculosis peptides at least once. M. tuberculosis epitope recognition by sarcoidosis patient sera was 42.7%, and by TB patient sera was 39.1%. Seven and 16 peptides were recognized in 9/12 (75%) and 8/12 (67%) sarcoidosis patient sera but not in TB patient sera, respectively. Nine (75%) and eight (67%) out of twelve TB patient sera, respectively recognized M. tuberculosis peptides that were not recognized in sarcoidosis patient sera. CONCLUSIONS: Specific IgG recognition patterns for M. tuberculosis antigens in sarcoidosis patients re-affirm mycobacterial involvement in sarcoidosis, providing biologically relevant targets for future studies pertaining to diagnostics and immunotherapy.
| Type: | Article |
|---|---|
| Title: | Humoral immune profiling of mycobacterial antigen recognition in sarcoidosis and Lofgren's syndrome using high-content peptide microarrays |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.ijid.2017.01.021 |
| Publisher version: | https://doi.org/10.1016/j.ijid.2017.01.021 |
| Language: | English |
| Additional information: | Copyright © 2017 Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Keywords: | Peptide microarray; Tuberculosis; Sarcoidosis; Humoral immune responses; tuberculosis antigens; Löfgren’s syndrome |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1551408 |
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