Zdora, M-C;
Vila-Comamala, J;
Schulz, G;
Khimchenko, A;
Hipp, A;
Cook, AC;
Dilg, D;
... Zanette, I; + view all
(2017)
X-ray phase microtomography with a single grating for high-throughput investigations of biological tissue.
Biomedical Optics Express
, 8
(2)
pp. 1257-1270.
10.1364/BOE.8.001257.
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Abstract
The high-throughput 3D visualisation of biological specimens is essential for studying diseases and developmental disorders. It requires imaging methods that deliver high-contrast, high-resolution volumetric information at short sample preparation and acquisition times. Here we show that X-ray phase-contrast tomography using a single grating can provide a powerful alternative to commonly employed techniques, such as high-resolution episcopic microscopy (HREM). We present the phase tomography of a mouse embryo in paraffin obtained with an X-ray single-grating interferometer at I13-2 Beamline at Diamond Light Source and discuss the results in comparison with HREM measurements. The excellent contrast and quantitative density information achieved non-destructively and without staining using a simple, robust setup make X-ray single-grating interferometry an optimum candidate for high-throughput imaging of biological specimens as an alternative for existing methods like HREM.
| Type: | Article |
|---|---|
| Title: | X-ray phase microtomography with a single grating for high-throughput investigations of biological tissue |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1364/BOE.8.001257 |
| Publisher version: | http://doi.org/10.1364/BOE.8.001257 |
| Language: | English |
| Additional information: | Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Optics, Radiology, Nuclear Medicine & Medical Imaging, Biochemistry & Molecular Biology, Resolution Episcopic Microscopy, Talbot Interferometry, Human Cerebellum, Contrast, Mouse, Tomography, Mechanisms, Embryos, Atlas, Tool |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science > Childrens Cardiovascular Disease UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1550130 |
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