Wilson, MSC;
Saiardi, A;
(2017)
Importance of Radioactive Labelling to Elucidate Inositol Polyphosphate Signalling.
TOPICS IN CURRENT CHEMISTRY
, 375
, Article 14. 10.1007/s41061-016-0099-y.
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Abstract
Inositol polyphosphates, in their water-soluble or lipid-bound forms, represent a large and multifaceted family of signalling molecules. Some inositol polyphosphates are well recognised as defining important signal transduction pathways, as in the case of the calcium release factor Ins(1,4,5)P3, generated by receptor activation-induced hydrolysis of the lipid PtdIns(4,5)P2 by phospholipase C. The birth of inositol polyphosphate research would not have occurred without the use of radioactive phosphate tracers that enabled the discovery of the “PI response”. Radioactive labels, mainly of phosphorus but also carbon and hydrogen (tritium), have been instrumental in the development of this research field and the establishment of the inositol polyphosphates as one of the most important networks of regulatory molecules present in eukaryotic cells. Advancements in microscopy and mass spectrometry and the development of colorimetric assays have facilitated inositol polyphosphate research, but have not eliminated the need for radioactive experimental approaches. In fact, such experiments have become easier with the cloning of the inositol polyphosphate kinases, enabling the systematic labelling of specific positions of the inositol ring with radioactive phosphate. This approach has been valuable for elucidating their metabolic pathways and identifying specific and novel functions for inositol polyphosphates. For example, the synthesis of radiolabelled inositol pyrophosphates has allowed the discovery of a new protein post-translational modification. Therefore, radioactive tracers have played and will continue to play an important role in dissecting the many complex aspects of inositol polyphosphate physiology. In this review we aim to highlight the historical importance of radioactivity in inositol polyphosphate research, as well as its modern usage.
| Type: | Article |
|---|---|
| Title: | Importance of Radioactive Labelling to Elucidate Inositol Polyphosphate Signalling |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s41061-016-0099-y |
| Publisher version: | http://dx.doi.org/10.1007/s41061-016-0099-y |
| Additional information: | © The Author(s) 2017. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
| Keywords: | Science & Technology, Physical Sciences, Chemistry, Multidisciplinary, Chemistry, Radioactivity, Inositol, Pyrophosphates, Metabolism, Phosphate, MESSENGER-RNA EXPORT, DIPHOSPHOINOSITOL PENTAKISPHOSPHATE, HEXAKISPHOSPHATE KINASE, 1,3,4-TRISPHOSPHATE 5/6-KINASE, 1,4,5-TRISPHOSPHATE 3-KINASE, SACCHAROMYCES-CEREVISIAE, PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE, PROTEIN PYROPHOSPHORYLATION, 5-PHOSPHATASE ACTIVITY, CONSERVED FAMILY |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1549537 |
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