Morais, M;
Nunes, JPM;
Karu, K;
Forte, N;
Benni, I;
Smith, MEB;
Caddick, S;
... Baker, JR; + view all
(2017)
Optimisation of the dibromomaleimide (DBM) platform for native antibody conjugation by accelerated post-conjugation hydrolysis.
Organic and Biomolecular Chemistry
, 15
(14)
pp. 2859-2860.
10.1039/c7ob00220c.
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Abstract
Disulfide bridging offers a convenient approach to generate site-selective antibody conjugates from native antibodies. To optimise the reagents available to achieve this strategy, we describe here the use of dibromomaleimides designed to undergo accelerated post-conjugation hydrolysis. Conjugation and hydrolysis, which serve to 'lock' the conjugates as robustly stable maleamic acids, is achieved in just over 1 h. This dramatic acceleration is also shown to infer significant improvements in homogeneity, as demonstrated by mass spectrometry analysis.
Type: | Article |
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Title: | Optimisation of the dibromomaleimide (DBM) platform for native antibody conjugation by accelerated post-conjugation hydrolysis |
Location: | England |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1039/c7ob00220c |
Publisher version: | http://dx.doi.org/10.1039/c7ob00220c |
Language: | English |
Additional information: | This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material. |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
URI: | https://discovery.ucl.ac.uk/id/eprint/1546855 |
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