Sanen, K;
Martens, W;
Georgiou, M;
Ameloot, M;
Lambrichts, M;
Phillips, JB;
(2017)
Engineered neural tissue with Schwann cell differentiated human dental pulp stem cells: potential for peripheral nerve repair?
Journal of Tissue Engineering and Regenerative Medicine
, 11
(12)
pp. 3362-3372.
10.1002/term.2249.
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Abstract
Despite the spontaneous regenerative capacity of the peripheral nervous system, large gap peripheral nerve injuries (PNIs) require bridging strategies. The limitations and suboptimal results obtained with autografts or hollow nerve conduits in the clinic urge the need for alternative treatments. Recently, we have described promising neuroregenerative capacities of Schwann cells derived from differentiated human dental pulp stem cells (d-hDPSCs) in vitro. Here, we extended the in vitro assays to show the pro-angiogenic effects of d-hDPSCs, such as enhanced endothelial cell proliferation, migration and differentiation. In addition, for the first time we evaluated the performance of d-hDPSCs in an in vivo rat model of PNI. Eight weeks after transplantation of NeuraWrap™ conduits filled with engineered neural tissue (EngNT) containing aligned d-hDPSCs in 15-mm rat sciatic nerve defects, immunohistochemistry and ultrastructural analysis revealed ingrowing neurites, myelinated nerve fibres and blood vessels along the construct. Although further research is required to optimize the delivery of this EngNT, our findings suggest that d-hDPSCs are able to exert a positive effect in the regeneration of nerve tissue in vivo.
| Type: | Article |
|---|---|
| Title: | Engineered neural tissue with Schwann cell differentiated human dental pulp stem cells: potential for peripheral nerve repair? |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/term.2249 |
| Publisher version: | http://dx.doi.org/10.1002/term.2249 |
| Language: | English |
| Additional information: | This is the peer reviewed version of the following article: Sanen, K; Martens, W; Georgiou, M; Ameloot, M; Lambrichts, M; Phillips, JB; (2017) Engineered neural tissue with Schwann cell differentiated human dental pulp stem cells: potential for peripheral nerve repair? Journal of Tissue Engineering and Regenerative Medicine, which has been published in final form at: http://dx.doi.org/10.1002/term.2249. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmacology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1537479 |
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