De Almeida, I;
Oliveira, NMM;
Randall, RA;
Hill, CS;
McCoy, JM;
Stern, CD;
(2017)
Calreticulin is a secreted BMP antagonist, expressed in Hensen's node during neural induction.
Developmental Biology
, 421
(2)
pp. 161-170.
10.1016/j.ydbio.2016.12.001.
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Abstract
Hensen's node is the “organizer” of the avian and mammalian early embryo. It has many functions, including neural induction and patterning of the ectoderm and mesoderm. Some of the signals responsible for these activities are known but these do not explain the full complexity of organizer activity. Here we undertake a functional screen to discover new secreted factors expressed by the node at this time of development. Using a Signal Sequence Trap in yeast, we identify several candidates. Here we focus on Calreticulin. We show that in addition to its known functions in intracellular Calcium regulation and protein folding, Calreticulin is secreted, it can bind to BMP4 and act as a BMP antagonist in vivo and in vitro. Calreticulin is not sufficient to account for all organizer functions but may contribute to the complexity of its activity.
| Type: | Article |
|---|---|
| Title: | Calreticulin is a secreted BMP antagonist, expressed in Hensen's node during neural induction |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.ydbio.2016.12.001 |
| Publisher version: | http://doi.org/10.1016/j.ydbio.2016.12.001 |
| Language: | English |
| Additional information: | © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/). |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Developmental Biology, Neural plate, Gastrulation, Neurulation, Chick embryo, Phospho-Smad, Calnexin, Calcium-binding proteins, CALCIUM-BINDING PROTEIN, ENDOPLASMIC-RETICULUM, SARCOPLASMIC-RETICULUM, CARDIAC DEVELOPMENT, SIGNAL PEPTIDES, GENE-EXPRESSION, FACTORS CHORDIN, QUALITY-CONTROL, CHICK-EMBRYO, XENOPUS |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1532459 |
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