Kaynezhad, P;
Tachtsidis, I;
Jeffery, G;
(2016)
Optical monitoring of retinal respiration in real time: 670 nm light increases the redox state of mitochondria.
Experimental Eye Research
, 152
pp. 88-93.
10.1016/j.exer.2016.09.006.
Preview |
Text
1-s2.0-S0014483516302809-main.pdf Download (896kB) | Preview |
Abstract
Mitochondria play a key role in ageing and disease. Their membrane potentials and ATP production decline with age and this is associated with progressive inflammation, cell loss and death. Here we use broadband Near-Infrared Spectroscopy (NIRS) to non-invasively measure in-vivo changes in aged retinal mitochondrial respiration following exposure to 670 nm, which improves mitochondrial performance and reduces inflammation. Low power NIR light was shone into the eye via a fibre optic and the reflection monitored to measure signature changes in the oxidation of cytochrome c oxidase (COX) in complex IV of the electron transport chain. Changes in retinal haemodynamics and oxygenation were also recorded simultaneously with COX by measuring changes in oxygenated and deoxygenated haemoglobin (Δ[HbO2] and Δ[HHb]). Retinae of aged rats exposed to 670 nm for 5 mins showed consistent progressive increases in oxidation of COX 5 mins post exposure. This remained significantly greater than baseline for up to 2 h. This was not seen when retinae were exposed to 420 nm light of the same power or when no light was applied. 670 nm exposure significantly increased total haemoglobin concentration (Δ[HbT] = Δ[HbO2] +Δ[HHb]) but not haemoglobin difference (Δ[HbDiff] = Δ[HbO2] -Δ[HHb]). There were no changes in blood metrics in association with 420 nm light or when no light exposure was given. Hence, brief 670 nm exposure that is associated with reduced inflammation has a significant positive impact on the redox state of COX in aged retinae. The relative redox state of retinal COX may provide a valuable biomarker in ageing and macular degeneration where declining mitochondrial function is implicated.
| Type: | Article |
|---|---|
| Title: | Optical monitoring of retinal respiration in real time: 670 nm light increases the redox state of mitochondria |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.exer.2016.09.006 |
| Publisher version: | http://dx.doi.org/10.1016/j.exer.2016.09.006 |
| Language: | English |
| Additional information: | © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Ophthalmology, Retinal imaging, Cytochrome c oxidase, Macular degeneration, NEAR-INFRARED SPECTROSCOPY, MACULAR DEGENERATION, BLUE-LIGHT, PHOTORECEPTOR, DAMAGE, Retinal imaging, Cytochrome c oxidase, Macular degeneration |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1520228 |
Archive Staff Only
 |
View Item |
