Baptist, M;
Panagabko, C;
Cockcroft, S;
Atkinson, J;
(2016)
Ligand and membrane-binding behavior of the phosphatidylinositol transfer proteins PITPα and β.
Biochemistry and Cell Biology
, 94
(6)
pp. 528-533.
10.1139/bcb-2015-0152.
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Baptist et al 2016 Ligand and membrane binding behaviour of PITPs (Biochemsitry and Cell Biology).pdf - Accepted Version Download (1MB) | Preview |
Abstract
Phosphatidylinositol transfer proteins (PITPs) are believed to be lipid transfer proteins due to their ability to transfer either PI or PC between membrane compartments in vitro. However, the detailed mechanism of this transfer process is not fully established. To further understand the transfer mechanism of PITPs we examined the interaction of PITPs with membranes using dual polarization interferometry (DPI) which measures protein binding affinity on a flat immobilized lipid surface. In addition, a fluorescence resonance energy transfer (FRET)-based assay was also employed to monitor how quickly PITPs transfer their ligands to lipid vesicles. DPI analysis revealed that PITPβ had a higher affinity to membranes compared to PITPα. Furthermore, the FRET-based transfer assay revealed that PITPβ has a higher ligand transfer rate compared to PITPα. However, both PITPα and PITPβ demonstrated a preference for highly curved membrane surfaces during ligand transfer. In other words, ligand transfer rate was higher when the accepting vesicles were highly curved.
| Type: | Article |
|---|---|
| Title: | Ligand and membrane-binding behavior of the phosphatidylinositol transfer proteins PITPα and β |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1139/bcb-2015-0152 |
| Publisher version: | http://dx.doi.org/10.1139/bcb-2015-0152 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1503620 |
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