Subrizi, F;
Cárdenas-Fernández, M;
Lye, GJ;
Ward, JM;
Dalby, PA;
Sheppard, TD;
Hailes, HC;
(2016)
Transketolase catalysed upgrading of l -arabinose: the one-step stereoselective synthesis of l -gluco-heptulose.
Green Chemistry
, 18
(10)
pp. 3158-3165.
10.1039/C5GC02660A.
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Abstract
Conversion of biomass using biocatalysis is likely to become a technology that contributes significantly to the future production of chemical building blocks, materials and transport fuels. Here the synthesis of a value-added chemical from L-arabinose, a major component of the carbohydrates in sugar beet pulp (SBP), in a concise and sustainable manner has been investigated. Biocatalytic conversions using transketolase variants have been developed for the efficient, scalable synthesis of a rare naturally occurring ketoheptose, L-gluco-heptulose, from L-arabinose. New active E. coli TK mutants that readily accept L-arabinose were identified using a versatile colorimetric screening assay and the reaction was performed on a preparative scale.
| Type: | Article |
|---|---|
| Title: | Transketolase catalysed upgrading of l -arabinose: the one-step stereoselective synthesis of l -gluco-heptulose |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/C5GC02660A |
| Publisher version: | http://dx.doi.org/10.1039/C5GC02660A |
| Language: | English |
| Additional information: | This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0) |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1495981 |
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