Shmueli, K;
Dodd, SJ;
Van Gelderen, P;
Duyn, JH;
(2017)
Investigating lipids as a source of chemical exchange-induced MRI frequency shifts.
NMR in Biomedicine
, 30
(4)
, Article e3525. 10.1002/nbm.3525.
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Abstract
While magnetic susceptibility is a major contributor to NMR resonance frequency variations in the human brain, a substantial contribution may come from the chemical exchange of protons between water and other molecules. Exchange-induced frequency shifts fe have been measured in tissue and protein solutions, but relatively lipid-rich white matter (WM) has a larger fe than gray matter, suggesting that lipids could contribute. Galactocerebrosides (GC) are a prime candidate as they are abundant in WM and susceptible to exchange. To investigate this, fe was measured in a model of WM lipid membranes in the form of multilamellar vesicles (MLVs), consisting of a 1:2 molar ratio of GC and phospholipids (POPC), and in MLVs with POPC only. Chemical shift imaging with 15% volume fraction of dioxane, an internal reference whose protons are assumed not to undergo chemical exchange, was used to remove susceptibility-induced frequency shifts in an attempt to measure fe in MLVs at several lipid concentrations. Initial analysis of these measurements indicated a necessity to correct for small unexpected variations in dioxane concentration due to its effect on the water frequency shift. To achieve this, the actual dioxane concentration was inferred from spectral analysis and its additional contribution to fe was removed through separate experiments which showed that the water–dioxane frequency shift depended linearly on the dioxane concentration at low concentrations with a proportionality constant of –0.021 ± 0.002 ppb/mM in agreement with published experiments. Contrary to expectations and uncorrected results, for GC + POPC vesicles, the dependence of the corrected fe on GC concentration was insignificant (0.023 ± 0.037 ppb/mM; r² = 0.085, p > 0.57), whereas for the POPC-only vesicles a small but significant linear increase with POPC concentration was found: 0.044 ± 0.008 ppb/mM (r² = 0.877, p < 0.01). These findings suggest that the exchange-induced contribution of lipids to frequency contrast in WM may be small.
| Type: | Article |
|---|---|
| Title: | Investigating lipids as a source of chemical exchange-induced MRI frequency shifts |
| Location: | UK |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/nbm.3525 |
| Publisher version: | http://dx.doi.org/10.1002/nbm.3525 |
| Language: | English |
| Additional information: | This is the peer reviewed version of the following article: Shmueli, K; Dodd, SJ; Van Gelderen, P; Duyn, JH; (2016) Investigating lipids as a source of chemical exchange-induced MRI frequency shifts, NMR in Biomedicine, which has been published in final form at http://dx.doi.org/10.1002/nbm.3525. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-820227.html#terms). |
| Keywords: | chemical exchange, exchange-induced resonance frequency shifts, chemical shift imaging, dioxane, multilamellar lipid vesicles, phospholipids, Galacto-Cerebrosides |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1482076 |
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