Numrich, J; Peli-Gulli, M-P; Arlt, H; Sardu, A; Griffith, J; Levine, T; Engelbrecht-Vandre, S; ... Ungermann, C; + view all Numrich, J; Peli-Gulli, M-P; Arlt, H; Sardu, A; Griffith, J; Levine, T; Engelbrecht-Vandre, S; Reggiori, F; De Virgilio, C; Ungermann, C; - view fewer (2015) The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis. Journal of Cell Science , 128 (13) pp. 2278-2292. 10.1242/jcs.164905.

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Abstract

Membrane fusion at the vacuole depends on a conserved machinery that includes SNAREs, the Rab7 homolog Ypt7 and its effector HOPS. Here, we demonstrate that Ypt7 has an unexpected additional function by controlling membrane homeostasis and nutrient-dependent signaling on the vacuole surface. We show that Ivy1, the yeast homolog of mammalian missing-in-metastasis (MIM), is a vacuolar effector of Ypt7-GTP and interacts with the EGO/ragulator complex, an activator of the target of rapamycin kinase complex 1 (TORC1) on vacuoles. Loss of Ivy1 does not affect EGO vacuolar localization and function. In combination with the deletion of individual subunits of the V-ATPase, however, we observed reduced TORC1 activity and massive enlargement of the vacuole surface. Consistent with this, Ivy1 localizes to invaginations at the vacuole surface and on liposomes in a phosphoinositide- and Ypt7-GTP-controlled manner, which suggests a role in microautophagy. Our data, thus, reveal that Ivy1 is a novel regulator of vacuole membrane homeostasis with connections to TORC1 signaling.

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