Numrich, J;
Peli-Gulli, M-P;
Arlt, H;
Sardu, A;
Griffith, J;
Levine, T;
Engelbrecht-Vandre, S;
... Ungermann, C; + view all
(2015)
The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis.
Journal of Cell Science
, 128
(13)
pp. 2278-2292.
10.1242/jcs.164905.
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Abstract
Membrane fusion at the vacuole depends on a conserved machinery that includes SNAREs, the Rab7 homolog Ypt7 and its effector HOPS. Here, we demonstrate that Ypt7 has an unexpected additional function by controlling membrane homeostasis and nutrient-dependent signaling on the vacuole surface. We show that Ivy1, the yeast homolog of mammalian missing-in-metastasis (MIM), is a vacuolar effector of Ypt7-GTP and interacts with the EGO/ragulator complex, an activator of the target of rapamycin kinase complex 1 (TORC1) on vacuoles. Loss of Ivy1 does not affect EGO vacuolar localization and function. In combination with the deletion of individual subunits of the V-ATPase, however, we observed reduced TORC1 activity and massive enlargement of the vacuole surface. Consistent with this, Ivy1 localizes to invaginations at the vacuole surface and on liposomes in a phosphoinositide- and Ypt7-GTP-controlled manner, which suggests a role in microautophagy. Our data, thus, reveal that Ivy1 is a novel regulator of vacuole membrane homeostasis with connections to TORC1 signaling.
| Type: | Article |
|---|---|
| Title: | The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1242/jcs.164905 |
| Publisher version: | http://dx.doi.org/10.1242/jcs.164905 |
| Language: | English |
| Additional information: | © 2015. Published by The Company of Biologists Ltd |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Cell Biology, Ivy1, Ypt7, Vps33, Torc1, EGO Complex, Vacuole Biogenesis, Yeast Saccharomyces-Cerevisiae, Hops Tethering Complex, Ego Complex, Autophagosome Formation, Nucleotide Exchange, Lysosomal Surface, Snare Chaperones, Homotypic Fusion, Binding Protein, Controls TORC1 |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1481385 |
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