Sellar, RS;
Fraser, L;
Khwaja, A;
Gale, RE;
Marafioti, T;
Akarca, A;
Hubank, M;
... Linch, DC; + view all
(2016)
Cell cycle status in AML blast cells from peripheral blood, bone marrow aspirates and trephines and implications for biological studies and treatment.
British Journal of Haematology
, 174
(2)
pp. 275-279.
10.1111/bjh.14055.
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Abstract
Using immunohistochemistry and flow cytometry to define phases of the cell cycle, this study shows that a high proportion of acute myeloid leukaemia (AML) blasts obtained from trephine biopsies are cycling, whereas >95% of peripheral blood-derived blasts are arrested in G1 . Results obtained from bone marrow aspirates are more similar to those from blood rather than from trephine biopsies. These differences were confirmed by gene expression profiling in a patient with high count AML. This has implications for cell cycle and other biological studies using aspirates rather than trephine biopsies and for the use of cell mobilising agents before chemotherapy.
| Type: | Article |
|---|---|
| Title: | Cell cycle status in AML blast cells from peripheral blood, bone marrow aspirates and trephines and implications for biological studies and treatment |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/bjh.14055 |
| Publisher version: | http://dx.doi.org/10.1111/bjh.14055 |
| Language: | English |
| Additional information: | This is the peer reviewed version of the following article: Sellar, RS; Fraser, L; Khwaja, A; Gale, RE; Marafioti, T; Akarca, A; Hubank, M; (2016) Cell cycle status in AML blast cells from peripheral blood, bone marrow aspirates and trephines and implications for biological studies and treatment. British Journal of Haematology, which has been published in final form at http://dx.doi.org/10.1111/bjh.14055. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving |
| Keywords: | AML, cell cycle, flow cytometry, gene expression, immunocytochemistry |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Pathology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1480901 |
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