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Development of a rapid, reliable and quantitative method: "SPOTi" for testing antifungal efficacy

Rizi, K; Murdan, S; Danquah, CA; Faull, J; Bhakta, S; (2015) Development of a rapid, reliable and quantitative method: "SPOTi" for testing antifungal efficacy. Journal of Microbiological Methods , 117 pp. 36-40. 10.1016/j.mimet.2015.07.005. Green open access

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Abstract

A reference method for the antimicrobial susceptibility testing of common fungal pathogens such as dermatophytes, is currently lacking. In this study, we report the successful adaptation of solid agar-based spot culture growth inhibition assay (SPOTi) for dermatophytes, currently being used as a gold-standard in the anti-tubercular drug discovery field. The fungal-SPOTi assay correlated with the disc-diffusion method, and is validated using mycelial plugs. We propose the fungal-SPOTi as a high-throughput alternative to the disc-diffusion and broth micro-dilution anti-fungal assays to screen novel anti-fungals.

Type: Article
Title: Development of a rapid, reliable and quantitative method: "SPOTi" for testing antifungal efficacy
Location: Netherlands
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.mimet.2015.07.005
Publisher version: http://dxd.oi.org/10.1016/j.mimet.2015.07.005
Additional information: © 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Antifungals, Dermatophytes, Drug discovery, SPOTi, Screening
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
URI: https://discovery.ucl.ac.uk/id/eprint/1476955
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