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A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase

Baud, D; Ladkau, N; Moody, TS; Ward, JM; Hailes, HC; (2015) A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase. Chemical Communications , 51 (97) pp. 17225-17228. 10.1039/c5cc06817g. Green open access

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Abstract

A new colorimetric method has been developed to screen transaminases using an inexpensive amine donor. The assay is sensitive, has a low level of background coloration, and can be used to identify and profile transaminase activities against aldehyde and ketone substrates in a high-throughput format. Significantly it is also amendable to solid phase colony screening.

Type: Article
Title: A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1039/c5cc06817g
Publisher version: http://dx.doi.org/10.1039/c5cc06817g
Language: English
Additional information: This journal is ©The Royal Society of Chemistry 2015. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/1474041
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