Baud, D;
Ladkau, N;
Moody, TS;
Ward, JM;
Hailes, HC;
(2015)
A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase.
Chemical Communications
, 51
(97)
pp. 17225-17228.
10.1039/c5cc06817g.
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Abstract
A new colorimetric method has been developed to screen transaminases using an inexpensive amine donor. The assay is sensitive, has a low level of background coloration, and can be used to identify and profile transaminase activities against aldehyde and ketone substrates in a high-throughput format. Significantly it is also amendable to solid phase colony screening.
Type: | Article |
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Title: | A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase |
Location: | England |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1039/c5cc06817g |
Publisher version: | http://dx.doi.org/10.1039/c5cc06817g |
Language: | English |
Additional information: | This journal is ©The Royal Society of Chemistry 2015. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
URI: | https://discovery.ucl.ac.uk/id/eprint/1474041 |
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