Fletcher, AJ;
Christensen, DE;
Nelson, C;
Tan, CP;
Schaller, T;
Lehner, PJ;
Sundquist, WI;
(2015)
TRIM5α requires Ube2W to anchor Lys63-linked ubiquitin chains and restrict reverse transcription.
EMBO Journal
, 34
(15)
pp. 2078-2095.
10.15252/embj.201490361.
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Abstract
TRIM5α is an antiviral, cytoplasmic, E3 ubiquitin (Ub) ligase that assembles on incoming retroviral capsids and induces their premature dissociation. It inhibits reverse transcription of the viral genome and can also synthesize unanchored polyubiquitin (polyUb) chains to stimulate innate immune responses. Here, we show that TRIM5α employs the E2 Ub-conjugating enzyme Ube2W to anchor the Lys63-linked polyUb chains in a process of TRIM5α auto-ubiquitination. Chain anchoring is initiated, in cells and in vitro, through Ube2W-catalyzed monoubiquitination of TRIM5α. This modification serves as a substrate for the elongation of anchored Lys63-linked polyUb chains, catalyzed by the heterodimeric E2 enzyme Ube2N/Ube2V2. Ube2W targets multiple TRIM5α internal lysines with Ub especially lysines 45 and 50, rather than modifying the N-terminal amino group, which is instead αN-acetylated in cells. E2 depletion or Ub mutation inhibits TRIM5α ubiquitination in cells and restores restricted viral reverse transcription, but not infection. Our data indicate that the stepwise formation of anchored Lys63-linked polyUb is a critical early step in the TRIM5α restriction mechanism and identify the E2 Ub-conjugating cofactors involved.
| Type: | Article |
|---|---|
| Title: | TRIM5α requires Ube2W to anchor Lys63-linked ubiquitin chains and restrict reverse transcription |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.15252/embj.201490361 |
| Publisher version: | http://dx.doi.org/10.15252/embj.201490361 |
| Additional information: | © 2015 The Authors. Published under the terms of the CC BY 4.0 license. This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | TRIM5α, Ube2N, Ube2W, restriction, ubiquitin, Carrier Proteins, HEK293 Cells, HeLa Cells, Humans, Models, Biological, Mutagenesis, Site-Directed, RNA Interference, RNA, Small Interfering, Reverse Transcription, Ubiquitin, Ubiquitin-Conjugating Enzymes |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1472626 |
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