Jones, GP;
Elliott, SJ;
Russell, IJ;
Lukashkin, AN;
(2015)
Modified Protein Expression in the Tectorial Membrane of the Cochlea Reveals Roles for the Striated Sheet Matrix.
Biophysical Journal
, 108
(1)
203 - 210.
10.1016/j.bpj.2014.11.1854.
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Abstract
The tectorial membrane (TM) of the mammalian cochlea is a complex extracellular matrix which, in response to acoustic stimulation, displaces the hair bundles of outer hair cells (OHCs), thereby initiating sensory transduction and amplification. Here, using TM segments from the basal, high-frequency region of the cochleae of genetically modified mice (including models of human hereditary deafness) with missing or modified TM proteins, we demonstrate that frequency-dependent stiffening is associated with the striated sheet matrix (SSM). Frequency-dependent stiffening largely disappeared in all three TM mutations studied where the SSM was absent either entirely or at least from the stiffest part of the TM overlying the OHCs. In all three TM mutations, dissipation of energy is decreased at low (<8 kHz) and increased at high (>8 kHz) stimulus frequencies. The SSM is composed of polypeptides carrying fixed charges, and electrostatic interaction between them may account for frequency-dependent stiffness changes in the material properties of the TM. Through comparison with previous in vivo measurements, it is proposed that implementation of frequency-dependent stiffening of the TM in the OHC attachment region facilitates interaction among tones, backward transmission of energy, and amplification in the cochlea.
| Type: | Article |
|---|---|
| Title: | Modified Protein Expression in the Tectorial Membrane of the Cochlea Reveals Roles for the Striated Sheet Matrix |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.bpj.2014.11.1854 |
| Publisher version: | http://dx.doi.org/10.1016/j.bpj.2014.11.1854 |
| Language: | English |
| Additional information: | © Elsevier 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. Access may be initially be restricted by the publisher |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1463536 |
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