Yusuf, Mohammed;
Millas, Ana Luiza G;
Estandarte, Ana Katrina C;
Bhella, Gurdeep K;
McKean, Robert;
Bittencourt, Edison;
Robinson, Ian K;
(2014)
Platinum blue staining of cells grown in electrospun scaffolds.
BioTechniques
, 57
(3)
pp. 137-141.
10.2144/000114206.
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Abstract
Fibroblast cells grown in electrospun polymer scaffolds were stained with platinum blue, a heavy metal stain, and imaged using scanning electron microscopy. Good contrast on the cells was achieved compared with samples that were gold sputter coated. The cell morphology could be clearly observed, and the cells could be distinguished from the scaffold fibers. Here we optimized the required concentration of platinum blue for imaging cells grown in scaffolds and show that a higher concentration causes platinum aggregation. Overall, platinum blue is a useful stain for imaging cells because of its enhanced contrast using scanning electron microscopy (SEM). In the future it would be useful to investigate cell growth and morphology using three-dimensional imaging methods.
| Type: | Article |
|---|---|
| Title: | Platinum blue staining of cells grown in electrospun scaffolds |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.2144/000114206 |
| Publisher version: | http://dx.doi.org/10.2144/000114206 |
| Language: | English |
| Additional information: | This is an open access article distributed under the terms of the Creative Commons CC BY license, which permits unrestricted use, distribution, reproduction in any medium, provided the original work is properly cited. You are not required to obtain permission to reuse this article in part or whole. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Chemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1458004 |
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