Huy, TQ;
Van Chung, P;
Thuy, NT;
Blanco-Andujar, C;
Thanh, NT;
(2014)
Protein A-conjugated iron oxide nanoparticles for separation of Vibrio cholerae from water samples.
Faraday Discuss
, 175
pp. 73-82.
10.1039/c4fd00152d.
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Abstract
Pathogen separation is of great significance for precise detection and prevention of disease outbreaks. For the first time, protein A conjugated with chitosan-coated iron oxide nanoparticles was prepared for pathogen separation at low concentrations from liquid samples. Vibrio cholerae O1 (VO1) bacteria were used for testing the effectiveness of this conjugate. Transmission electron microscopy (TEM) was used to confirm the presence of captured VO1. The results showed that, after binding with a specific antibody, the conjugate allows separation of VO1 bacteria from water samples at a concentration as low as 10 cfu mL(-1). Moreover, the conjugate can be used in parallel with conventional or modern diagnostic tests for quick and accurate detection of pathogens.
| Type: | Article |
|---|---|
| Title: | Protein A-conjugated iron oxide nanoparticles for separation of Vibrio cholerae from water samples. |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/c4fd00152d |
| Publisher version: | http://dx.doi.org/10.1039/c4fd00152d |
| Language: | English |
| Additional information: | This article is licensed under a Creative Commons Attribution 3.0 Unported Licence |
| Keywords: | Bacteriological Techniques, Ferric Compounds, Nanoparticles, Particle Size, Staphylococcal Protein A, Surface Properties, Vibrio cholerae, Water Microbiology |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1448056 |
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