Mannion, I;
(2007)
Role of PTEN in regulation of cell migration in glioma cells.
Masters thesis , UCL (University College London).
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Abstract
The Phosphatase and Tensin Homologue deleted on Chromosome 10 (PTEN) is a protein and lipid phosphatase whose activity is crucial for tumour suppression. The most notable activity of PTEN is as a phosphatase of the signalling lipid phosphatidylinositol-3,4,5-trisphosphate (PIP3), and thus as an antagonist of the phosphatidylinositol-3-kinase (PI3K) signalling pathway. Loss of PTEN activity in cells is often an important step in tumourigenesis. In addition to its catalytic site, PTEN also contains a C2 domain, which is able to inhibit cell migration in human glioma cell lines (Raftopoulou et al., 2004). This thesis examines the effect of expression of PTEN and its C2 domain on various aspects of cellular behaviour in human U373 glioma cells. Firstly, it is demonstrated that the PTEN C2 domain shares no significant primary sequence homology with other human proteins, but that its structure is remarkably similar to that of other human C2 domains. Secondly, it is shown that PTEN-C2 is able to interact specifically with a number of inositol phospholipids. Thirdly, phosphorylation at Thr383 is shown to modulate sub-cellular localisation of PTEN. Finally, it is demonstrated that expression of full-length PTEN or the isolated C2 domain is able to perturb the morphology and polarity of U373 glioma cells.
| Type: | Thesis (Masters) |
|---|---|
| Title: | Role of PTEN in regulation of cell migration in glioma cells |
| Identifier: | PQ ETD:594132 |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | Thesis digitised by ProQuest. Third party copyright material has been removed from the ethesis. Images identifying individuals have been redacted or partially redacted to protect their identity. |
| UCL classification: | |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1446383 |
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