Chang, J.H.; (2006) Adhesion patterns of Neisseria lactamica and its implications. Doctoral thesis , University of London.

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Abstract

Neisseria Meningitidis is a pathogenic bacteria responsible for a wide range of serious disease, including septsis and meningitis especially in children and infants. In contrast Neisseria lac/arnica which resides m the same ecological niche as N.meningitidis, the nasopharynx, does not cause disease and is commensal. It was found that the two species of Neisseria have converse carriage rates where N.lactamica has a protecthe role <ner N.meningitidis colonization in infants. The two species have similar structural components and genes but very different pathogenesis. It is clear that N.meningitidis adheres and invades host cells whereas N.lactamica does not invade. Adhesion of N.lactamica seems to occur mostly around the sides of the cell rather than the apex or base. Adhesion numbers of N.lactamica increase with time of infection with a greater increase from 4 to 6 hours rather than 2 to 4 hours of infection. Adhesion rates in relation to N.meningitidis adhesion numbers were less which may be dye to greater adhesive ability of As meningitidis. Inhibitor treatment of epithelial cells and thus, a decrease in cellular inflammatory response, resulted in agre-Mei J gree of adhesion and again from 4 to 6 hours, the increase in adhesion numbers mere vi eater than from. 2 to 4 hours. Supernatant quantifications showed an increase in N.Lietantiea with time,which may reflect growth of bacteria dining the course of infection. Further experimentations with advanced techniques such as con focal microscopy and more repeats of the gentamycin assay would yield more promising results. Also, the analysis of supernatant for inflammatory mediators, bactericidal compounds, and bacterial by products is necessary in order to determine the dynamics of adhesion of N.lactamica.

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