de Bruin, EC;
van de Pas, S;
Lips, EH;
van Eijk, R;
van der Zee, MM;
Lombaerts, M;
van Wezel, T;
... Peltenburg, LT; + view all
(2005)
Macrodissection versus microdissection of rectal carcinoma: minor influence of stroma cells to tumor cell gene expression profiles.
BMC Genomics
, 6
, Article 142. 10.1186/1471-2164-6-142.
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Abstract
The molecular determinants of carcinogenesis, tumor progression and patient prognosis can be deduced from simultaneous comparison of thousands of genes by microarray analysis. However, the presence of stroma cells in surgically excised carcinoma tissues might obscure the tumor cell-specific gene expression profiles of these samples. To circumvent this complication, laser microdissection can be performed to separate tumor epithelium from the surrounding stroma and healthy tissue. In this report, we compared RNAs isolated from macrodissected, of which only surrounding healthy tissue had been removed, and microdissected rectal carcinoma samples by microarray analysis in order to determine the most reliable approach to detect the expression of tumor cell-derived genes by microarray analysis.
| Type: | Article |
|---|---|
| Title: | Macrodissection versus microdissection of rectal carcinoma: minor influence of stroma cells to tumor cell gene expression profiles |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1186/1471-2164-6-142 |
| Publisher version: | http://dx.doi.org/10.1186/1471-2164-6-142 |
| Language: | English |
| Additional information: | © 2005 de Bruin et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. PMCID: PMC1283972 |
| Keywords: | Carcinoma, Cluster Analysis, DNA, Complementary, Gene Expression Profiling, Gene Expression Regulation, Gene Expression Regulation, Neoplastic, Humans, Microarray Analysis, Microdissection, RNA, RNA, Messenger, RNA, Neoplasm, Rectal Neoplasms, Software, Stromal Cells |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1424493 |
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