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Rational substrate and enzyme engineering of transketolase for aromatics

Payongsri, P; Steadman, D; Strafford, J; MacMurray, A; Hailes, HC; Dalby, PA; (2012) Rational substrate and enzyme engineering of transketolase for aromatics. Organic & Biomolecular Chemistry , 10 (45) 9021 - 9029. 10.1039/c2ob25751c. Green open access

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Abstract

The uses of 3-formylbenzoic acid and 4-formylbenzoic acid as molecular probes along with previous and new transketolase mutants revealed the factors governing the rate of reaction between transketolase and aromatic aldehydes. The novel α,α-dihydroxyketones were produced at 15 to 30-fold higher yields and up to 250-fold higher specific activities with D469T TK when compared to those obtained for benzaldehyde.

Type: Article
Title: Rational substrate and enzyme engineering of transketolase for aromatics
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1039/c2ob25751c
Publisher version: http://dx.doi.org/10.1039/c2ob25751c
Language: English
Keywords: Benzoic Acid, Catalytic Domain, Escherichia coli, Hydrogen Bonding, Kinetics, Molecular Docking Simulation, Mutation, Protein Engineering, Static Electricity, Transketolase
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/1417400
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