Payongsri, P;
Steadman, D;
Strafford, J;
MacMurray, A;
Hailes, HC;
Dalby, PA;
(2012)
Rational substrate and enzyme engineering of transketolase for aromatics.
Organic & Biomolecular Chemistry
, 10
(45)
9021 - 9029.
10.1039/c2ob25751c.
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Abstract
The uses of 3-formylbenzoic acid and 4-formylbenzoic acid as molecular probes along with previous and new transketolase mutants revealed the factors governing the rate of reaction between transketolase and aromatic aldehydes. The novel α,α-dihydroxyketones were produced at 15 to 30-fold higher yields and up to 250-fold higher specific activities with D469T TK when compared to those obtained for benzaldehyde.
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