Chittka, A;
(2013)
Differential regulation of SC1/PRDM4 and PRMT5 mediated protein arginine methylation by the nerve growth factor and the epidermal growth factor in PC12 cells.
Neuroscience Letters
, 550
87 - 92.
10.1016/j.neulet.2013.06.051.
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Abstract
During neuronal development, the neuroepithelial stem cells (NSCs) initially undergo proliferative divisions, later switching to neurogenic ones whereby one NSC and a post-mitotic neuron are generated. We recently showed that a member of the PRDM family of transcriptional regulators, PRDM4/SC1, recruits a type II protein arginine methyltransferase, PRMT5, to maintain the "stem-like" cellular state of the embryonic mouse cortical NSCs. However, little is known about the regulation of activity of this complex under proliferation- or differentiation-inducing growth conditions. In the present work I investigate the regulation of SC1/PRMT5-mediated methylation activity in PC12 cells treated with EGF or NGF. I present evidence that NGF down-regulates SC1/PRMT5 methyltransferase (MTase) activity and that the reduction in SC1/PRMT5 MTase activity occurs mainly in the nucleus. I suggest that high levels of SC1/PRMT5 activity are associated with the proliferative state of the cells. © 2013 The Author.
| Type: | Article |
|---|---|
| Title: | Differential regulation of SC1/PRDM4 and PRMT5 mediated protein arginine methylation by the nerve growth factor and the epidermal growth factor in PC12 cells |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.neulet.2013.06.051 |
| Publisher version: | http://dx.doi.org/10.1016/j.neulet.2013.06.051 |
| Additional information: | © 2013 The Author. Published by Elsevier Ireland Ltd. All rights reserved. This is an open-access article distributed under the terms of the CreativeCommons Attribution License, which permits unrestricted use, distribution andreproduction in any medium, provided the original author and source are credited. |
| Keywords: | PRDM4; PRMT5; Histone arginine methylation; PC12 cells; NGF; |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Wolfson Inst for Biomedical Research |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1404803 |
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