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Binding specificity of the G1/S transcriptional regulators in budding yeast

Harris, MR; Lee, D; Farmer, S; Lowndes, NF; de Bruin, RA; (2013) Binding specificity of the G1/S transcriptional regulators in budding yeast. PLoS One , 8 (4) , Article e61059. 10.1371/journal.pone.0061059. Green open access

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[thumbnail of Table 1. Yeast strains used in this study] MS PowerPoint (Table 1. Yeast strains used in this study)
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[thumbnail of Figure 1. Polyclonal antisera generated against G1/S transcription factor components.] MS PowerPoint (Figure 1. Polyclonal antisera generated against G1/S transcription factor components.)
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[thumbnail of Figure 2. Nrm1 is a component of MBF and Whi5 of SBF.] MS PowerPoint (Figure 2. Nrm1 is a component of MBF and Whi5 of SBF.)
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[thumbnail of Figure 3. Mbp1 and Swi4 bind and regulate the RNR1 and CLN2 promoters respectively.] MS PowerPoint (Figure 3. Mbp1 and Swi4 bind and regulate the RNR1 and CLN2 promoters respectively.)
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[thumbnail of Figure 4. Dynamic changes of SBF target gene regulation during the cell cycle.] MS PowerPoint (Figure 4. Dynamic changes of SBF target gene regulation during the cell cycle.)
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[thumbnail of Figure 5. Model of SBF transcriptional regulation.] MS PowerPoint (Figure 5. Model of SBF transcriptional regulation.)
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Abstract

G1/S transcriptional regulation in the budding yeast Saccharomyces cerevisiae depends on three main transcriptional components, Swi4, Swi6 and Mbp1. These proteins constitute two transcription factor complexes that regulate over 300 G1/S transcripts, namely SBF (Swi4-Swi6) and MBF (Mbp1-Swi6). SBF and MBF are involved in regulating largely non-overlapping sets of G1/S genes via clearly distinct mechanisms.

Type: Article
Title: Binding specificity of the G1/S transcriptional regulators in budding yeast
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0061059
Publisher version: http://dx.doi.org/10.1371/journal.pone.0061059
Language: English
Additional information: PMCID: PMC3617184 © 2013 Harris et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Cyclins, DNA-Binding Proteins, G1 Phase, Gene Expression Regulation, Fungal, Promoter Regions, Genetic, Protein Binding, Protein Interaction Mapping, Repressor Proteins, Ribonucleotide Reductases, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
URI: https://discovery.ucl.ac.uk/id/eprint/1399972
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