Depledge, DP;
Palser, AL;
Watson, SJ;
Lai, IY;
Gray, ER;
Grant, P;
Kanda, RK;
... Breuer, J; + view all
(2011)
Correction: specific capture and whole-genome sequencing of viruses from clinical samples.
PLOS One
, 7
(1)
, Article e27805. 10.1371/annotation/3f1444bc-ab9d-4112-958a-2e068792f26f.
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Abstract
There was an error in the accession number in Materials and Methods. EBV and KSHV dataset can be found under the Study Accession number: ERP001026. Individual Sample Accessions are as follows: 1208_JSC1_SN_KS - ERS074823 1212_HBL6_SN_KS - ERS074824 1210_JSC1_SN_EB - ERS074825 1215_HBL6_SN_EB - ERS074833 Whole genome sequencing of viruses directly from clinical samples is integral for understanding the genetics of host-virus interactions. Here, we report the use of sample sparing target enrichment (by hybridisation) for viral nucleic acid separation and deep-sequencing of herpesvirus genomes directly from a range of clinical samples including saliva, blood, virus vesicles, cerebrospinal fluid, and tumour cell lines. We demonstrate the effectiveness of the method by deep-sequencing 13 highly cell-associated human herpesvirus genomes and generating full length genome alignments at high read depth. Moreover, we show the specificity of the method enables the study of viral population structures and their diversity within a range of clinical samples types.
| Type: | Article |
|---|---|
| Title: | Correction: specific capture and whole-genome sequencing of viruses from clinical samples. |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1371/annotation/3f1444bc-ab9d-4112-958a-2e068792f26f |
| Publisher version: | http://dx.doi.org/10.1371/journal.pone.0027805 |
| Language: | English |
| Additional information: | © 2011 Depledge et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. This work was supported by the Medical Research Centre [G0900950], Wellcome Trust [WT081703MA] and the European Community's Seventh Framework Programme [FP7/2007–2013] under the project EMPERIE, EC grant agreement number 223498. DPD is funded by the Medical Research Council Centre for Molecular Medical Virology [G07008], JB and ERG receive funding from the UCL/UCLH National Institute for Health Research Comprehensive Biomedical Research Centre. PK, ALP, SJW and IY-CL are funded by the Wellcome Trust Sanger Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| Keywords: | Cell Line, Tumor, DNA, Viral, Genome, Viral, Herpesviridae, Herpesviridae Infections, Herpesvirus 3, Human, Herpesvirus 4, Human, Herpesvirus 8, Human, Humans, Mutation, Polymerase Chain Reaction, Reproducibility of Results, Saliva, Sequence Analysis, DNA, Species Specificity |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1385503 |
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