Cashmore, TJ;
Peter, JG;
van Zyl-Smit, RN;
Semple, PL;
Maredza, A;
Meldau, R;
Zumla, A;
... Dheda, K; + view all
(2010)
Feasibility and Diagnostic Utility of Antigen-Specific Interferon-gamma Responses for Rapid Immunodiagnosis of Tuberculosis Using Induced Sputum.
PLOS ONE
, 5
(4)
, Article e10389. 10.1371/journal.pone.0010389.
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Abstract
Background: The diagnosis of smear-negative or sputum-scarce tuberculosis (TB) is problematic as culture takes several weeks and representative biological samples are difficult to obtain. RD-1 antigen-specific interferon-c release assays (IGRAs) are sensitive and specific blood-based tests for the diagnosis of M. tuberculosis infection. The feasibility and diagnostic utility of this rapid immunodiagnostic assay, using cells from induced sputum, is unknown.Methodology/Principal Findings: Cells isolated from induced sputum were co-cultured with ESAT-6 and CFP-10 antigens using a standardized enzyme-linked immunospot (ELISPOT) assay (T-SPOT (R).TB) in 101 consecutively recruited TB suspects or non-TB controls. An optimization phase using 28 samples was followed by a validation phase using samples from 73 participants (20 with definite or probable TB, and 48 with non-TB). Despite optimization of sputum processing 65/73 (89%) of the IGRAs in the validation phase were inconclusive. 44/73 (60%) tests failed due to sputum induction-related factors [sputum induction-related adverse events (n = 5), inadequate sputum volume (n = 8), non-homogenisable sputum (n = 7), and insufficient numbers of cells to perform the assay (n = 24)], whilst 20/73 (27%) tests failed due T-SPOT (R).TB assay-related factors [excessive debris precluding reading of spots in the ELISPOT well (n = 6), failure of the positive control (n = 11), or high spot count in the negative control (n = 3)]. Only 8/73 (11%) of the available samples could therefore be correctly categorized (7 definite or probable TB, and 1 non-TB patient). Thus, 13/20 (65%) of the definite or probable TB cases remained undiagnosed.Conclusions/Significance: Rapid immunodiagnosis of pulmonary TB by antigen-specific IFN-gamma ELISPOT responses, using cells from induced sputum, is possible. However, the test, in its current ELISPOT format, is not clinically useful because the majority of the assays are inconclusive.
| Type: | Article |
|---|---|
| Title: | Feasibility and Diagnostic Utility of Antigen-Specific Interferon-gamma Responses for Rapid Immunodiagnosis of Tuberculosis Using Induced Sputum |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1371/journal.pone.0010389 |
| Publisher version: | http://dx.doi.org/10.1371/journal.pone.0010389 |
| Language: | English |
| Additional information: | © 2010 Cashmore et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. This study was supported by the National Research Foundation (NRF) and a TBSusgent grant from the European Commission (EU-FP7). JP is supported by a SATBAT award, a Discovery Foundation Fellowship and by the Fogarty International Clinical Research Scholars/Fellows Support Centre National Institutes of Health grant R24TW007988. RVZS is supported by a Discovery Foundation Fellowship and by the Fogarty International Clinical Research Scholars/Fellows Support Centre NIH grant R24TW007988. KD is supported by a TBsusgent grant from the European Commission (EU-FP7), the EDCTP, a SA MRC Career Development Award, a Canadian CIHR award, and a NRF/SARChI award. Prof. A. Zumla receives support from EU (TrDNA), EuropeAID (ADAT), EDCTP, MRC (UK) and the NIHR UCLH-CBRC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| Keywords: | SMEAR-NEGATIVE TUBERCULOSIS, PULMONARY TUBERCULOSIS, BRONCHOSCOPY, INDUCTION |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery.ucl.ac.uk/id/eprint/113832 |
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