Lane, Majella;
(2025)
Wound healing and photodynamic potential of Adiantum capillus-veneris L. (Pteridaceae): An evaluation of the cellular effects and molecular insights.
Frontiers in Pharmacology
10.3389/fphar.2025.1729572.
(In press).
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Abstract
Chronic and infected wounds represent a persistent global health burden. Medicinal plants offer a promising source of wound-healing agents due to their multitarget activities, long history of traditional use, and accessibility. Adiantum capillus-veneris L. (ACV), traditionally used to treat a range of ailments, such as respiratory, urinary, and skin disorders, was investigated for its in vitro wound-healing potential following methanol extraction. We evaluated the effects of methanol extracts of ACV (ACVM) on cell proliferation, migration and antioxidative capacity in human keratinocytes (HaCaT), and anti-inflammatory activity in RAW 264.7 cells. We also explored its combination with visible light phototherapy. Chemical profiling via HPTLC analysis, UV/Vis spectrophotometry and HPLC analysis, together confirmed that ACVM contained more metabolites than other extracts, yielding five visible-light absorption peaks and identifying rutin and chlorogenic acid as major metabolites. At ≤100 µg/mL, ACVM was non-toxic to HaCaT cells in the absence of visible light. However, phototoxicity was evident at 200 µg/mL. ACVM (50 µg/mL) significantly promoted HaCaT migration, with a further 2 enhancement upon exposure to light. ACVM also suppressed H₂O₂-induced ROS generation in a dose-dependent manner (≤50 µg/mL), while light exerted a bidirectional modulatory effect. Additionally, ACVM markedly inhibited LPS-induced secretion of CXCL2, CCL2, CXCL10, TNF-α, and IL-6 in RAW 264.7 macrophages, with effects evident at concentrations as low as 0.1 µg/mL. These findings suggest that ACVM, particularly in combination with light-assisted therapy, shows promise for accelerating wound healing.
| Type: | Article |
|---|---|
| Title: | Wound healing and photodynamic potential of Adiantum capillus-veneris L. (Pteridaceae): An evaluation of the cellular effects and molecular insights |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fphar.2025.1729572 |
| Publisher version: | http://dx.doi.org/10.3389/fphar.2025.1729572 |
| Language: | English |
| Additional information: | Copyright: © 2025 Mou, Jalil, Scotti, Lane, Fiebich, Sun, Maake and Heinrich. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| Keywords: | Adiantum capillus-veneris L., medicinal plants, Photodynamic therapy (PDT), Skin, Wound Healing |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10218080 |
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