Islam, Mohammad Mirazul;
Saha, Amrita;
Trisha, Farzana Afrose;
Gonzalez-Andrades, Miguel;
Patra, Hirak K;
Griffith, May;
Chodosh, James;
(2024)
An in vitro 3-dimensional Collagen-based Corneal Construct with Innervation Using Human Corneal Cell Lines.
Ophthalmology Science
, 4
(6)
, Article 100544. 10.1016/j.xops.2024.100544.
Preview |
Text
Patra_1-s2.0-S2666914524000800-main.pdf Download (1MB) | Preview |
Abstract
Purpose: To develop a 3-dimensional corneal construct suitable for in vitro studies of disease conditions and therapies. Design: In vitro human corneal constructs were created using chemically crosslinked collagen and chondroitin sulfate extracellular matrix and seeded with 3 human corneal cell types (epithelial, stromal, and endothelial) together with neural cells. The neural cells were derived from hybrid neuroblastoma cells and the other cells used from immortalized human corneal cell lines. To check the feasibility and characterize the constructs, cytotoxicity, cell proliferation, histology, and protein expression studies were performed. Results: Optimized culture condition permitted synchronized viability across the cell types within the construct. The construct showed a typical appearance for different cellular layers, including healthy appearing, phenotypically differentiated neurons. The expected protein expression profiles for specific cell types within the construct were confirmed with western blotting. Conclusions: An in vitro corneal construct was successfully developed with maintenance of individual cell phenotypes with anatomically correct cellular loci. The construct may be useful in evaluation of specific corneal disorders and in developing different corneal disease models. Additionally, the construct can be used in evaluating drug targeting and/or penetration to individual corneal layers, testing novel therapeutics for corneal diseases, and potentially reducing the necessity for animals in corneal research at the early stages of investigation. Financial Disclosure(s): Proprietary or commercial disclosure may be found in the Footnotes and Disclosures at the end of this article.
| Type: | Article |
|---|---|
| Title: | An in vitro 3-dimensional Collagen-based Corneal Construct with Innervation Using Human Corneal Cell Lines |
| Location: | Netherlands |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.xops.2024.100544 |
| Publisher version: | https://doi.org/10.1016/j.xops.2024.100544 |
| Language: | English |
| Additional information: | ª 2024 by the American Academy of Ophthalmology This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Published by Elsevier Inc. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Ophthalmology, In vitro 3D corneal construct, Corneal extracellular matrix, Crosslinking, EXTRACELLULAR-MATRIX, EPITHELIAL-CELLS, DIFFERENTIATION, PERMEATION, MODELS, RECONSTRUCTION, EQUIVALENTS, MAINTENANCE, CULTURES, HEALTH |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10217462 |
Archive Staff Only
 |
View Item |
