Jeong, Bosu;
Yoon, Ji-Young;
Ahn, Junyong;
Lee, Byunghak;
Park, Sung-Min;
Kim, Jae-Heon;
Bayarkhangai, Buuvee;
... Lee, Jung-Hwan; + view all
(2025)
Eco-Fabricated Nanowave-Textured Implants Drive Microtubule-Assisted Nuclear Mechanotransduction and Chromatin Modification: Biophysical Priming for Osteogenesis and Bone Regeneration.
Advanced Functional Materials
, Article e03422. 10.1002/adfm.202503422.
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Adv Funct Materials - 2025 - Jeong - Eco%E2%80%90Fabricated Nanowave%E2%80%90Textured Implants Drive Microtubule%E2%80%90Assisted Nuclear.pdf - Published Version Download (19MB) | Preview |
Abstract
The biophysical cues from implantable materials, specifically nanotopography, play a pivotal role in directing cellular lineage specification, thereby accelerating tissue healing and regeneration. Despite the recognized impact of these cues, the mechanisms governing mechano‐activated signaling pathways between the cytoskeletal and nuclear domains remain largely unexplored. Here, the processes underlying the enhanced osteogenesis of mesenchymal stem cells (MSCs) driven by nanotextured implants are elucidated, focusing on alterations in cytoskeletal mechanosensitive molecules and nuclear chromatin structures. Using green‐processed femtosecond laser fabrication, an implant platform featuring nanowave textures is engineered, inducing cellular alignment with oriented cytoskeletons and consequential changes in nuclear shape. Notably, activated and aligned microtubules alongside the nucleus play a key role in shaping nuclear morphology. The nanowave textures induce significant modifications in chromatin structure, characterized by increased histone acetylation, implying a mechano‐priming of MSCs for osteogenesis. Mechanically‐primed MSCs exhibit enhanced osteogenic transcriptional responsiveness to biochemical cues, with mechanosensitive YAP co‐signaling with the RUNX2, facilitated by an opened chromatin structure. In vivo experiments in a rabbit tibia reveal that nanowave‐textured implants promote osteogenesis and bone formation. This study underscores the ability of nanowave‐textured cues to transmit mechano‐signals across the cytoskeletal‐to‐nuclear space in MSCs, leading to stimulated osteogenesis.
| Type: | Article |
|---|---|
| Title: | Eco-Fabricated Nanowave-Textured Implants Drive Microtubule-Assisted Nuclear Mechanotransduction and Chromatin Modification: Biophysical Priming for Osteogenesis and Bone Regeneration |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/adfm.202503422 |
| Publisher version: | https://doi.org/10.1002/adfm.202503422 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons License. The images or other third-party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Science & Technology, Physical Sciences, Technology, Chemistry, Multidisciplinary, Chemistry, Physical, Nanoscience & Nanotechnology, Materials Science, Multidisciplinary, Physics, Applied, Physics, Condensed Matter, Chemistry, Science & Technology - Other Topics, Materials Science, Physics, eco-friendly, femtosecond laser, nanotopography, nuclear mechanosensing, osteogenesis, CELL, SURFACE, NANOTOPOGRAPHY, DIFFERENTIATION, TENSION, YAP |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10211934 |
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