Dudziak, Diana;
Heger, Lukas;
Agace, William W;
Bakker, Joyce;
De Gruijl, Tanja D;
Dress, Regine J;
Dutertre, Charles-Antoine;
... Zelle-Rieser, Claudia; + view all
(2025)
Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC.
European Journal of Immunology
, 55
(1)
, Article 2250325. 10.1002/eji.202250325.
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Abstract
This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs, and various nonlymphoid tissues. Within this article, detailed protocols are presented that allow for the generation of single-cell suspensions from human nonlymphoid tissues including lung, skin, gingiva, intestine as well as from tumors and tumor-draining lymph nodes with a subsequent analysis of dendritic cells by flow cytometry. Further, prepared single-cell suspensions can be subjected to other applications including cellular enrichment procedures, RNA sequencing, functional assays, etc. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.
| Type: | Article |
|---|---|
| Title: | Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC |
| Location: | Germany |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/eji.202250325 |
| Publisher version: | https://doi.org/10.1002/eji.202250325 |
| Language: | English |
| Additional information: | Copyright © 2024 The Author(s). European Journal of Immunology published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, https://creativecommons.org/licenses/by/4.0/, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Dendritic cells; nonlymphoid tissues; tumor; tumor-draining lymph node; flow cytometry |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10210871 |
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