Greig, Alan;
Henrique, Rui;
Xie, Boyu;
Thrasivoulou, Christopher;
Millar, Michael;
Ahmed, Aamir;
(2025)
Super-Resolution Microscopy Reveals Alterations in Clathrin Structure in Human Cancer Tissue.
The American Journal of Pathology
, 195
(9)
pp. 1619-1626.
10.1016/j.ajpath.2025.05.008.
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Abstract
Super-resolution microscopy holds great promise for detailed structural analysis of proteins, yet its application in the investigations of protein structures in situ remains sparse. Clathrin-coated pit-mediated endocytosis (CME) plays a key role in human cancer. This study aimed to discover whether there are structural changes in clathrin pits in cancer. Immunofluorescence combined with super-resolution structured illumination microscopy (SR-SIM) on normal and cancerous prostate tissue was used to reveal novel details of clathrin structure and biology. Clathrin (heavy-chain) plaques and pits, expression of adaptor protein 2 (a clathrin adaptor protein), and epidermal growth factor receptor (a receptor target for CME) at nanometer scale in human tissue were observed in situ with immunofluorescence-SR-SIM. The size of the clathrin pits in high-grade cancer was greater compared with that in low-grade or normal prostate tissue. These results demonstrate that SR-SIM can be used to identify protein structures at high resolution in clinical tissue sections and there is an increased cargo capacity due to the increase in the size of clathrin pits as a mechanism that facilitates aggressiveness of cancer. These results shed new light on the pathology of cancer and the role CME via clathrin may play in carcinogenesis.
| Type: | Article |
|---|---|
| Title: | Super-Resolution Microscopy Reveals Alterations in Clathrin Structure in Human Cancer Tissue |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.ajpath.2025.05.008 |
| Publisher version: | https://doi.org/10.1016/j.ajpath.2025.05.008 |
| Language: | English |
| Additional information: | Copyright © 2025 American Society for Investigative Pathology. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10209019 |
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