Kravvas, G;
Boyu, X;
Ganier, C;
Van den Munckhof, H;
Van den Munckhof, E;
De Koning, MNC;
Gulin, SJ;
... Bunker, CB; + view all
(2025)
A direct comparative analysis of HPV DNA with single molecule RNA and p16INK4a protein expression in lichen sclerosus: implications for diagnostics and pathogenesis.
JID Innovations
, Article 100367. 10.1016/j.xjidi.2025.100367.
(In press).
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Abstract
Introduction: Emerging evidence suggests a relationship between chronic, intermittent, occluded exposure of a susceptible epithelium to urine and male genital lichen sclerosus (MGLSc), though human papillomavirus (HPV) may also play a role. / Aims & Methods: This study investigated the association between MGLSc and HPV across the prepuce. Preputial samples from uncircumcised patients with MGLSc undergoing circumcision were tested for MGLSc distribution, HPV genotyping, RNAscope, and p16INK4a detection. / Results: Preputial samples from nine MGLSc patients were analyzed, with nine distinct areas per prepuce, yielding 81 samples. These included MGLSc, non-MGLSc, and indeterminate regions. Various mucosal and beta HPV types were detected, most commonly HPV24, HPV23, HPV36, and HPV9. HPV DNA was found in all patients, and high-risk HPV types in six. No significant differences were observed in total HPV (p=0.1) or oncogenic HPV (p=0.6) between MGLSc and non-MGLSc tissues. Transcriptionally active HPV was absent in all samples based on independent RNAscope and p16INK4a staining. / Discussion: HPV DNA was detected in a mosaic pattern across the prepuce, with no significant differences between MGLSc and non-MGLSc skin. The absence of transcriptional activity suggests HPV in MGLSc is incidental and may not contribute towards pathogenesis.
| Type: | Article |
|---|---|
| Title: | A direct comparative analysis of HPV DNA with single molecule RNA and p16INK4a protein expression in lichen sclerosus: implications for diagnostics and pathogenesis |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.xjidi.2025.100367 |
| Publisher version: | https://doi.org/10.1016/j.xjidi.2025.100367 |
| Language: | English |
| Additional information: | This is an Open Access article published under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Licence (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Keywords: | Papillomavirus, Gene Transcription, Inflammatory Skin Diseases, RNA Biology, Transcription |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10207247 |
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