Liu, Sai;
Weston, Andrew;
Satta, Giovanni;
Bolognini, Sara;
Di Luca, Mariagrazia;
Gaisford, Simon;
Williams, Gareth R;
(2025)
Anti-Pseudomonas aeruginosa phage-loaded electrosprayed lactose particles.
Journal of Drug Delivery Science and Technology
10.1016/j.jddst.2025.106851.
(In press).
Preview |
Text
Weston_Anti-Pseudomonas aeruginosa phage-loaded electrosprayed lactose particles_Pre-proof.pdf Download (1MB) | Preview |
Abstract
In light of the growing issue of antibiotic resistance, this study explored phage encapsulation within lactose particles for potential use in treating oral and ear bacterial infections. Firstly, phage-loaded suspensions that ensured phage stability at RT for 24 h were developed. After electrospraying, phage-loaded particles were successfully generated with an encapsulation efficiency (EE) of 31% at a phage stock concentration of 50% v/v. The phage titer decreased only slightly from 5 × 107 PFU/mg to 1.5 × 107 PFU/mg after processing, maintaining the same order of magnitude. The particles exhibited spherical morphology and successful phage encapsulation, with a burst release of phages within 10 minutes of immersion in an aqueous medium (99.0 ± 6.9%). The phage-loaded particles demonstrated enhanced antibacterial efficacy compared to pure phage solutions, completely inhibiting P. aeruginosa growth for over 24 h at a particle concentration of 20 mg/ml, likely due to a high localized phage concentration. Stability studies indicated that phages remained viable at various temperatures, with greater stability at lower temperatures (4°C, and -20°C), though phage activity gradually decreased over time in particle form, particularly at room temperature.
Loading...
Loading...Loading...| 1. |  Europe | 1 |
Archive Staff Only
 |
View Item |
