Armstrong, Alexander;
(2025)
Effect of temperature on the purification of monoclonal antibodies using protein A, cation exchange, and anion exchange chromatography.
Doctoral thesis (Ph.D), UCL (University College London).
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Abstract
Temperature is an under-characterized process parameter in the chromatographic purification of mAbs. The goal of this PhD thesis was to further the understanding of the effects of temperature on both batch and continuous chromatography purification of mAbs using protein A and anion exchange resins. The primary objectives of this work are as follows: 1) Develop a temperature-controlled batch and 3-column counter-current protein A system for improved therapeutic purification. 2) Determine the effect of temperature on batch adsorption and thermodynamics of mAbs on anion chromatography resin. 3) Determine the effect of temperature on resin lifetime due to resin degradation caused by the loading of HCCF material on protein A resin. The research has produced two published research papers and a third in pre-print. A fourth publication is planned. In addition, three conference talks and two poster presentations were given. As part of the CODOBIO grant and the collaboration with the UCL-AstraZeneca Center of Excellence, multiple yearly and semi-yearly presentations were given by the PhD candidate which helped to strengthen his oratory skills. The PhD candidate benefited greatly from his studies at UCL and he is now working at Amgen in Thousand Oaks, California, USA, as a Senior Scientist in early stage downstream process development.
| Type: | Thesis (Doctoral) |
|---|---|
| Qualification: | Ph.D |
| Title: | Effect of temperature on the purification of monoclonal antibodies using protein A, cation exchange, and anion exchange chromatography |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | Copyright © The Author 2025. Original content in this thesis is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) Licence (https://creativecommons.org/licenses/by-nc/4.0/). Any third-party copyright material present remains the property of its respective owner(s) and is licensed under its existing terms. Access may initially be restricted at the author’s request. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10205477 |
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