Xu, R;
Brookshaw, T;
Erro, E;
Roberts, M;
Selden, C;
Martin, E;
(2024)
Device Design for the Rapid Ultrasonic Rewarming of Alginate Beads and Cryoprotectant Solution to Improve Cryopreservation Recovery.
In:
IEEE Ultrasonics, Ferroelectrics, and Frequency Control Joint Symposium, UFFC-JS 2024 - Proceedings.
(pp. pp. 1-6).
IEEE
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Abstract
Ultrasonic rewarming is a new method for improving cryopreservation recovery. We developed an ultrasonic rewarming device and control algorithm for the rapid and repeatable rewarming of cryopreserved biological media in cryovials. We characterised the device's acoustic output with hydrophone measurements and rewarming rates with thermocouple measurements. Mean rewarming rates of 120°C/min were achieved with cryopreserved alginate beads and cryoprotectant solution. The ultrasound exposures implemented free-field pressure amplitudes in the 3 MPa range, corresponding to 100 W electrical absorbed power. The exposure duration timing was accurate to ~0.5 s. The device will provide a platform for ultrasonic rewarming cell viability studies.
| Type: | Proceedings paper |
|---|---|
| Title: | Device Design for the Rapid Ultrasonic Rewarming of Alginate Beads and Cryoprotectant Solution to Improve Cryopreservation Recovery |
| Event: | 2024 IEEE Ultrasonics, Ferroelectrics, and Frequency Control Joint Symposium (UFFC-JS), 22-26 September 2024, Taipei, Taiwan |
| Dates: | 22 Sep 2024 - 26 Sep 2024 |
| ISBN-13: | 979-8-3503-7190-1 |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1109/UFFC-JS60046.2024.10794129 |
| Publisher version: | https://doi.org/10.1109/uffc-js60046.2024.10794129 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. - This work was supported by a UKRI Future Leaders Fellowship (Grant No. MR/T019166/1), and by the Wellcome/EPSRC Centre for Interventional and Surgical Sciences (WEISS) (203145Z/16/Z). The author has applied for a CC BY public copyright licence to any manuscript version arising from this submission. |
| Keywords: | Focused Ultrasound, Rewarming, Device Design |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inst for Liver and Digestive Hlth |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10204996 |
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