Taylor, Emerald R;
Sato, Akiko;
Jones, Isobel;
Gudeangadi, Prashant G;
Beal, David M;
Hopper, James A;
Xue, Wei-Feng;
... Serpell, Christopher J; + view all
(2023)
Tuning dynamic DNA- and peptide-driven selfassembly in DNA–peptide conjugates.
Chemical Science
, 14
(1)
pp. 196-202.
10.1039/D2SC02482A.
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Abstract
DNA–peptide conjugates offer an opportunity to marry the benefits of both biomolecular classes, combining the high level of programmability found with DNA, with the chemical diversity of peptides. These hybrid systems offer potential in fields such as therapeutics, nanotechnology, and robotics. Using the first DNA–β-turn peptide conjugate, we present three studies investigating the self-assembly of DNA–peptide conjugates over a period of 28 days. Time-course studies, such as these have not been previously conducted for DNA–peptide conjugates, although they are common in pure peptide assembly, for example in amyloid research. By using aging studies to assess the structures produced, we gain insights into the dynamic nature of these systems. The first study explores the influence varying amounts of DNA–peptide conjugates have on the self-assembly of our parent peptide. Study 2 explores how DNA and peptide can work together to change the structures observed during aging. Study 3 investigates the presence of orthogonality within our system by switching the DNA and peptide control on and off independently. These results show that two orthogonal self-assemblies can be combined and operated independently or in tandem within a single macromolecule, with both spatial and temporal effects upon the resultant nanostructures.
| Type: | Article |
|---|---|
| Title: | Tuning dynamic DNA- and peptide-driven selfassembly in DNA–peptide conjugates |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/D2SC02482A |
| Publisher version: | https://doi.org/10.1039/D2SC02482A |
| Language: | English |
| Additional information: | © The Author(s), 2022. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/3.0/ |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10204799 |
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